Objectives To explore serum cytokines levels (including IL-1β, sIL-2R, IL-6, TNF-α, and IFN-ν) and their significance in patients with acute coronary syndrome (ACS) and the subsequent follow-ups, with attempt to es...Objectives To explore serum cytokines levels (including IL-1β, sIL-2R, IL-6, TNF-α, and IFN-ν) and their significance in patients with acute coronary syndrome (ACS) and the subsequent follow-ups, with attempt to estimate the role of various serum inflammatory markers in the diagnosis and assessment of ACS. Methods The study population include 40 patients with acute myocardial infarction (AMI), 40 patients with unstable angina pectoris (UAP), and 40 controls. Among the 80 patients, 60 patients attended a follow up 4 months later. Serum inflammatory markers including IL-1β, sIL-2R, IL-6, TNF-α, and IFN-νwere measured by enzyme linked immunosorbent assay. ResultsSerum IL-1β, sIL-2R, IL-6, TNF-αwere significantly higher in AMI group or UAP group compared to the con-trol group and became significantly lower 4 months later in the follow-up patients. Serum levels of IFN-νshows no signifi-cant difference between AMI group or UAP group and controls, also showing no significant change when measured in follow up patients. There was no correlation between serum creatine kinase-MB isoenzyme levels and serum inflammatory markers either in UAP or AMI group. Furthermore, when divided into two subgroups using Wagner’s QRS scoring system in the AMI group, there is no difference of each serum inflammatory marker between ≤6 scores group and > 6 scores group. Conclusion Serum levels of certain inflammatory markers may have some diagnostic value for ACS, and can be a us-eful marker reflecting disease stability.展开更多
Objective： To study whether the anti-AS effect of ginkgo biloba extract （GbE） was related with inhibitory effects on the expression of IL-1β, TNF-α and up-regulation of IL-10 and IL-10R in the heart of atheroscle...Objective： To study whether the anti-AS effect of ginkgo biloba extract （GbE） was related with inhibitory effects on the expression of IL-1β, TNF-α and up-regulation of IL-10 and IL-10R in the heart of atherosclerotic （AS） rats. Methods： The experimental model of AS rats were established by intraperitioneal injection of vitamin D3 with high fat and cholesterol diet. All rats were divided into 3 groups： control, AS and GbE. GbE （100 mg/kg） was administered to rats by ig. After 8 weeks, the expression of IL-1β, TNF-α, IL-10, and IL-10R in the heart of AS rats were detected by enzyme-linked immunosorbant assay, reverse transcriptase polymerasechain reaction and Western blotting. Results： The protein and mRNA expressions of IL-1β, TNF-α, IL-10 and IL-10R were markedly higher in AS group than those in control group （P〈0.01）. The protein and mRNA expressions of IL-1β and TNF-α were markedly lower in GbE group than those in AS group; while the protein and mRNA expressions of IL-10 and IL-10R were markedly higher in GbE group than those in AS group （P〈0.01）. Conclusion： GbE has significant inhibitory effects on proinflammatory cytokine IL-1β, TNF-α. The up-regulation of anti-inflammatory cytokine IL-10, IL-10R that may he partially responsible for its anti-AS effects.展开更多
文摘Objectives To explore serum cytokines levels (including IL-1β, sIL-2R, IL-6, TNF-α, and IFN-ν) and their significance in patients with acute coronary syndrome (ACS) and the subsequent follow-ups, with attempt to estimate the role of various serum inflammatory markers in the diagnosis and assessment of ACS. Methods The study population include 40 patients with acute myocardial infarction (AMI), 40 patients with unstable angina pectoris (UAP), and 40 controls. Among the 80 patients, 60 patients attended a follow up 4 months later. Serum inflammatory markers including IL-1β, sIL-2R, IL-6, TNF-α, and IFN-νwere measured by enzyme linked immunosorbent assay. ResultsSerum IL-1β, sIL-2R, IL-6, TNF-αwere significantly higher in AMI group or UAP group compared to the con-trol group and became significantly lower 4 months later in the follow-up patients. Serum levels of IFN-νshows no signifi-cant difference between AMI group or UAP group and controls, also showing no significant change when measured in follow up patients. There was no correlation between serum creatine kinase-MB isoenzyme levels and serum inflammatory markers either in UAP or AMI group. Furthermore, when divided into two subgroups using Wagner’s QRS scoring system in the AMI group, there is no difference of each serum inflammatory marker between ≤6 scores group and > 6 scores group. Conclusion Serum levels of certain inflammatory markers may have some diagnostic value for ACS, and can be a us-eful marker reflecting disease stability.
基金Supported by the National Natural Science Foundation of China (No. 30271509)
文摘Objective： To study whether the anti-AS effect of ginkgo biloba extract （GbE） was related with inhibitory effects on the expression of IL-1β, TNF-α and up-regulation of IL-10 and IL-10R in the heart of atherosclerotic （AS） rats. Methods： The experimental model of AS rats were established by intraperitioneal injection of vitamin D3 with high fat and cholesterol diet. All rats were divided into 3 groups： control, AS and GbE. GbE （100 mg/kg） was administered to rats by ig. After 8 weeks, the expression of IL-1β, TNF-α, IL-10, and IL-10R in the heart of AS rats were detected by enzyme-linked immunosorbant assay, reverse transcriptase polymerasechain reaction and Western blotting. Results： The protein and mRNA expressions of IL-1β, TNF-α, IL-10 and IL-10R were markedly higher in AS group than those in control group （P〈0.01）. The protein and mRNA expressions of IL-1β and TNF-α were markedly lower in GbE group than those in AS group; while the protein and mRNA expressions of IL-10 and IL-10R were markedly higher in GbE group than those in AS group （P〈0.01）. Conclusion： GbE has significant inhibitory effects on proinflammatory cytokine IL-1β, TNF-α. The up-regulation of anti-inflammatory cytokine IL-10, IL-10R that may he partially responsible for its anti-AS effects.
