药物相关性颌骨坏死(medication-related osteonecrosis of the jaw,MRONJ)及放射性颌骨坏死(osteoradionecrosis of the jaw,ORNJ)是两种常见的难治性医源性颌骨坏死。随着骨组织工程的迅猛发展,将生物材料负载干细胞所制成的干细胞复...药物相关性颌骨坏死(medication-related osteonecrosis of the jaw,MRONJ)及放射性颌骨坏死(osteoradionecrosis of the jaw,ORNJ)是两种常见的难治性医源性颌骨坏死。随着骨组织工程的迅猛发展,将生物材料负载干细胞所制成的干细胞复合物,用于对MRONJ及ORNJ的预防和治疗成为本领域研究的重点和热点。本文通过对干细胞复合物防治MRONJ和ORNJ的相关研究进行回顾,阐述干细胞复合物对MRONJ及ORNJ的防治效果和影响因素,分析该技术有待探究的问题,以期为进一步研究指明方向,为临床应用提供依据。展开更多
Objective：To compare the phenotype characteristics of rat annulus fibrosus （AF） cells cultured on flexible silicone membranes and those in plastic plates. Methods：The morphology of AF cells cultured in different s...Objective：To compare the phenotype characteristics of rat annulus fibrosus （AF） cells cultured on flexible silicone membranes and those in plastic plates. Methods：The morphology of AF cells cultured in different suhstrates was examined. Proteoglycan was stained by toluidine blue. Contents of collagen type Ⅰ, collagen type Ⅰ and aggrecan mRNAs were determined by reverse transcription-polymerase chain reaction （RT-PCR）. The expression of integrin β1 was monitored by flow cytometry. By using propidium iodide （PI）, the cell cycle in AF cells was analyzed. Cell adhesion to silicone membrane was also measured. Results：The AF cells cultured on different suhstrates were morphologically undistinguishable. Toluidine blue staining showed that there was also no difference between AF cells cultured on these 2 substrates. They still had the same expression levels of collagen type Ⅰ , collagen type Ⅰ, aggrecan mRNAs, and integrin β1. No significant difference was observed in the distribution of the cell cycle. AF cells grew well on silicone membrane. Conclusion：AF cells cultured on flexible silicone membrane maintain the stability of phenotype and may he appropriate for further studying the metabolic responses to mechanical stimuli at the cellular level.展开更多
文摘药物相关性颌骨坏死(medication-related osteonecrosis of the jaw,MRONJ)及放射性颌骨坏死(osteoradionecrosis of the jaw,ORNJ)是两种常见的难治性医源性颌骨坏死。随着骨组织工程的迅猛发展,将生物材料负载干细胞所制成的干细胞复合物,用于对MRONJ及ORNJ的预防和治疗成为本领域研究的重点和热点。本文通过对干细胞复合物防治MRONJ和ORNJ的相关研究进行回顾,阐述干细胞复合物对MRONJ及ORNJ的防治效果和影响因素,分析该技术有待探究的问题,以期为进一步研究指明方向,为临床应用提供依据。
文摘Objective：To compare the phenotype characteristics of rat annulus fibrosus （AF） cells cultured on flexible silicone membranes and those in plastic plates. Methods：The morphology of AF cells cultured in different suhstrates was examined. Proteoglycan was stained by toluidine blue. Contents of collagen type Ⅰ, collagen type Ⅰ and aggrecan mRNAs were determined by reverse transcription-polymerase chain reaction （RT-PCR）. The expression of integrin β1 was monitored by flow cytometry. By using propidium iodide （PI）, the cell cycle in AF cells was analyzed. Cell adhesion to silicone membrane was also measured. Results：The AF cells cultured on different suhstrates were morphologically undistinguishable. Toluidine blue staining showed that there was also no difference between AF cells cultured on these 2 substrates. They still had the same expression levels of collagen type Ⅰ , collagen type Ⅰ, aggrecan mRNAs, and integrin β1. No significant difference was observed in the distribution of the cell cycle. AF cells grew well on silicone membrane. Conclusion：AF cells cultured on flexible silicone membrane maintain the stability of phenotype and may he appropriate for further studying the metabolic responses to mechanical stimuli at the cellular level.
