目的探讨纳米银离子对细菌生物被膜(biofilm,BF)的细菌死亡率的影响。方法采用摇床法,以纳米银离子含量不同的乙烯-醋酸乙烯酯(Ethylene-Vinyl acetate,EVA)塑料为细菌粘附载体,模拟体内铜绿假单胞菌(P.aerugi-nosa,PA)BF形成的微环境,...目的探讨纳米银离子对细菌生物被膜(biofilm,BF)的细菌死亡率的影响。方法采用摇床法,以纳米银离子含量不同的乙烯-醋酸乙烯酯(Ethylene-Vinyl acetate,EVA)塑料为细菌粘附载体,模拟体内铜绿假单胞菌(P.aerugi-nosa,PA)BF形成的微环境,建立体外BF模型;将培养3 d的空白标本分别在扫描电子显微镜(SEM)下及用FITC-ConA染色后荧光显微镜下观察不含纳米银离子EVA中BF的形成情况;将生长0.5、1、2、3、5 d的BF模型行SYTO9/PI染色,激光共聚焦扫描电镜(CLSM)下摄取不同层面的图像,然后采用Image Pro Plus 6.0分析软件分析不同干预条件下BF的细菌的死亡率。结果运用SEM及荧光显微镜的方法,在以不含纳米银离子EVA塑料的细菌粘附载体上培养3 d的标本中均观察到流线状的BF形成;纳米银离子含量、作用时间对BF的细菌死亡率均有明显影响(F值分别为84.62,85.67,P<0.01);不同时间点含有纳米银离子材料的BF细菌死亡率均显著高于空白对照组(P<0.05),但纳米银离子含量不同的2种材料上BF的细菌死亡率无明显影响(P>0.05);纳米银离子含量不同的(0、0.05%、0.1%)材料上的BF,其第1、2、3天细菌死亡率均分别显著高于第0.5、5天的细菌死亡率(P<0.05);含纳米银离子0.1%的材料上BF在作用的第2天其细菌死亡率最高[(88.53±1.88)%]。结论运用摇床法成功建立了体外BF模型,纳米银离子对BF内的细菌有明显杀灭作用。展开更多
Sm^(3+)-doped materials exhibit red and orange emissions in the visible light region,showing broad applica⁃tion prospects in both laser and display material fields.However,the inherent small emission and absorption cr...Sm^(3+)-doped materials exhibit red and orange emissions in the visible light region,showing broad applica⁃tion prospects in both laser and display material fields.However,the inherent small emission and absorption cross-sections of Sm^(3+)result in low luminous efficiency,posing challenges for achieving high-quality solid-state lighting.Here,the excellent white emission of Sm^(3+)doped lithium aluminum silicate(LAS)glass was realized by introducing the Ag aggregates through Ag ion exchange.Under 395 nm excitation,the Ag-doped samples exhibit significant fluo⁃rescence enhancement with color coordinates close to the equal energy white point E(0.33,0.33)and a color ren⁃dering index(CRI)of 81.8.The study reveals that the surface plasmon resonance(SPR)effect of Ag nanoparticles enhances the luminescence of Sm^(3+),while the energy transfer mechanism between Ag^(+)and Sm^(3+)also promotes fluores⁃cence enhancement.By adjusting the concentration of AgNO_(3) and the exchange time,a series of high-quality full-spectrum white light emissions were obtained,indicating that the Ag ion-exchanged Sm^(3+)-doped LAS glass has good application potential in the development of solid-state lighting devices.Moreover,variations in the excitation wave⁃length can effectively tune the emission color,further demonstrating the tunability and practicality of this material in optoelectronic applications.展开更多
文摘目的探讨纳米银离子对细菌生物被膜(biofilm,BF)的细菌死亡率的影响。方法采用摇床法,以纳米银离子含量不同的乙烯-醋酸乙烯酯(Ethylene-Vinyl acetate,EVA)塑料为细菌粘附载体,模拟体内铜绿假单胞菌(P.aerugi-nosa,PA)BF形成的微环境,建立体外BF模型;将培养3 d的空白标本分别在扫描电子显微镜(SEM)下及用FITC-ConA染色后荧光显微镜下观察不含纳米银离子EVA中BF的形成情况;将生长0.5、1、2、3、5 d的BF模型行SYTO9/PI染色,激光共聚焦扫描电镜(CLSM)下摄取不同层面的图像,然后采用Image Pro Plus 6.0分析软件分析不同干预条件下BF的细菌的死亡率。结果运用SEM及荧光显微镜的方法,在以不含纳米银离子EVA塑料的细菌粘附载体上培养3 d的标本中均观察到流线状的BF形成;纳米银离子含量、作用时间对BF的细菌死亡率均有明显影响(F值分别为84.62,85.67,P<0.01);不同时间点含有纳米银离子材料的BF细菌死亡率均显著高于空白对照组(P<0.05),但纳米银离子含量不同的2种材料上BF的细菌死亡率无明显影响(P>0.05);纳米银离子含量不同的(0、0.05%、0.1%)材料上的BF,其第1、2、3天细菌死亡率均分别显著高于第0.5、5天的细菌死亡率(P<0.05);含纳米银离子0.1%的材料上BF在作用的第2天其细菌死亡率最高[(88.53±1.88)%]。结论运用摇床法成功建立了体外BF模型,纳米银离子对BF内的细菌有明显杀灭作用。
文摘Sm^(3+)-doped materials exhibit red and orange emissions in the visible light region,showing broad applica⁃tion prospects in both laser and display material fields.However,the inherent small emission and absorption cross-sections of Sm^(3+)result in low luminous efficiency,posing challenges for achieving high-quality solid-state lighting.Here,the excellent white emission of Sm^(3+)doped lithium aluminum silicate(LAS)glass was realized by introducing the Ag aggregates through Ag ion exchange.Under 395 nm excitation,the Ag-doped samples exhibit significant fluo⁃rescence enhancement with color coordinates close to the equal energy white point E(0.33,0.33)and a color ren⁃dering index(CRI)of 81.8.The study reveals that the surface plasmon resonance(SPR)effect of Ag nanoparticles enhances the luminescence of Sm^(3+),while the energy transfer mechanism between Ag^(+)and Sm^(3+)also promotes fluores⁃cence enhancement.By adjusting the concentration of AgNO_(3) and the exchange time,a series of high-quality full-spectrum white light emissions were obtained,indicating that the Ag ion-exchanged Sm^(3+)-doped LAS glass has good application potential in the development of solid-state lighting devices.Moreover,variations in the excitation wave⁃length can effectively tune the emission color,further demonstrating the tunability and practicality of this material in optoelectronic applications.
