In order to produce macromolecules by using the immobilized cells in microcapsules, a novel macro-porous NaCS-PDMDAAC capsules system was used to immobilize Candida valida in a semi-continuous culture mode for produci...In order to produce macromolecules by using the immobilized cells in microcapsules, a novel macro-porous NaCS-PDMDAAC capsules system was used to immobilize Candida valida in a semi-continuous culture mode for producing lipase. It was found that the encapsulation system had no negative effect on the growth and lipase productivity, and the lipase produced inside capsules could diffuse freely to the media outside capsules. The cultivation with the capsules was carried out for 15 batches and the lipase in each batch had almost the same activity. In comparison with free cell cultivation, the immobilized cell fermentation shortened obviously the period of glucose consumption from 24 hours to 8 hours and increased lipase productivity.展开更多
A new type of NaCS/PDMDAAC polyelectrolyte complex membrane prepared by interfacial reaction of sodium cellulose sulfate as polyanions and poly(dimethylallylammonium chloride)as polycations was proposed.The static con...A new type of NaCS/PDMDAAC polyelectrolyte complex membrane prepared by interfacial reaction of sodium cellulose sulfate as polyanions and poly(dimethylallylammonium chloride)as polycations was proposed.The static contact angle of the polyelectrolyte complex(PELC)membrane surface was measured to characterize membrane wettability.The effects of concentration of polyelectrolyte,molecular weight,and reaction time on contact angle were investigated.It was found that NaCS/PDMDAAC PELC membrane was hydrophilic.Its contact angle decreased with the increasing concentration of NaCS and molecular weight of PDMDAAC,and increased with the increasing concentration of PDMDAAC and reaction time.展开更多
文摘In order to produce macromolecules by using the immobilized cells in microcapsules, a novel macro-porous NaCS-PDMDAAC capsules system was used to immobilize Candida valida in a semi-continuous culture mode for producing lipase. It was found that the encapsulation system had no negative effect on the growth and lipase productivity, and the lipase produced inside capsules could diffuse freely to the media outside capsules. The cultivation with the capsules was carried out for 15 batches and the lipase in each batch had almost the same activity. In comparison with free cell cultivation, the immobilized cell fermentation shortened obviously the period of glucose consumption from 24 hours to 8 hours and increased lipase productivity.
文摘A new type of NaCS/PDMDAAC polyelectrolyte complex membrane prepared by interfacial reaction of sodium cellulose sulfate as polyanions and poly(dimethylallylammonium chloride)as polycations was proposed.The static contact angle of the polyelectrolyte complex(PELC)membrane surface was measured to characterize membrane wettability.The effects of concentration of polyelectrolyte,molecular weight,and reaction time on contact angle were investigated.It was found that NaCS/PDMDAAC PELC membrane was hydrophilic.Its contact angle decreased with the increasing concentration of NaCS and molecular weight of PDMDAAC,and increased with the increasing concentration of PDMDAAC and reaction time.
