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用PCR检测牛精液中牛Ⅰ型疱疹病毒
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作者 李凯年 《畜牧兽医科技信息》 1997年第24期6-6,共1页
荷兰的研究人员研制了一种PCR测定法,用以检测选择性消化全牛精液中的牛Ⅰ型疱疹病霉(BHV-1)DNA。使用一种蛋白酶-K进行的简单处理以溶解游离病毒、在非精子细胞中存在的病毒及粘附于精子表面的病毒。这种处理不能使基因组牛DNA释放出... 荷兰的研究人员研制了一种PCR测定法,用以检测选择性消化全牛精液中的牛Ⅰ型疱疹病霉(BHV-1)DNA。使用一种蛋白酶-K进行的简单处理以溶解游离病毒、在非精子细胞中存在的病毒及粘附于精子表面的病毒。这种处理不能使基因组牛DNA释放出来。根据gD基因的核苷酸顺序选择引物和探针。用眼观溴乙锭染色的琼脂糖凝胶来检测PCR产物。 展开更多
关键词 牛Ⅰ型疱疹病毒 牛精液 PCR检测 PCR测定法 精子细胞 DNA释放 单处理 游离病毒 试验感染 精子表面
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Influence of surfactants on bioleaching of arsenic-containing gold concentrate 被引量:1
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作者 方芳 钟宏 +3 位作者 江放明 李朝辉 陈永发 湛雪辉 《Journal of Central South University》 SCIE EI CAS 2014年第10期3963-3969,共7页
To shorten the bioleaching cycle of arsenic-containing gold concentrate, surfactants were used to promote the interaction between bacteria and ore to increase the arsenic leaching rate. Three different kinds of surfac... To shorten the bioleaching cycle of arsenic-containing gold concentrate, surfactants were used to promote the interaction between bacteria and ore to increase the arsenic leaching rate. Three different kinds of surfactants were used to evaluate the effects of surfactants on the growth of bacteria and arsenic leaching rate of arsenic-containing gold concentrate. The mechanism underlying surfactant enhancement was also studied. Results show that when relatively low-concentration surfactants are added to the medium, no significant difference is observed in the growth and Fe2+ oxidation ability of the bacteria compared with no surfactant in the medium. However, only the anionic surfactant calcium lignosulfonate and the nonionic surfactant Tween 80 are found to improve the arsenic leaching rates. Their optimum mass concentrations are 30 and 80 mg/L, respectively. At such optimum mass concentrations, the arsenic leaching rates are approximately 13.7% and 9.1% higher than those without the addition of surfactant, respectively. Mechanism research reveals that adding the anionic surfactant calcium lignosulfonate improves the percentage of bacterial adhesion on the mineral surface and decreases the surface tension in the leaching solution. 展开更多
关键词 Acidithiobacillus ferrooxidans arsenic-containing gold concentrate SURFACTANTS BIOLEACHING
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