研究辣椒素(capsaicin,CAP)对高糖高脂饮食(high-fructose and high-fat diet,HFFD)喂养小鼠肝脏与胰腺葡萄糖代谢的昼夜节律调节作用。结果表明,CAP缓解了HFFD诱导的小鼠胰岛素抵抗,并增加了肝脏的糖原含量。CAP恢复了肝脏和和胰腺中...研究辣椒素(capsaicin,CAP)对高糖高脂饮食(high-fructose and high-fat diet,HFFD)喂养小鼠肝脏与胰腺葡萄糖代谢的昼夜节律调节作用。结果表明,CAP缓解了HFFD诱导的小鼠胰岛素抵抗,并增加了肝脏的糖原含量。CAP恢复了肝脏和和胰腺中生物钟基因和葡萄糖代谢相关基因的节律性表达,改善了HFFD小鼠的胰腺功能障碍,并重编程了其粪便代谢物。这些发现表明CAP可能作为一种功能性食品改善葡萄糖代谢的昼夜节律,同时为预防代谢紊乱和昼夜节律失调提供了更有效的饮食干预策略。展开更多
To investigate the in vitro digestion and fermentation properties of soybean oligosaccharides(SBOS)extracted from defatted soybean meal,the changes in monosaccharide composition and molecular mass were analyzed.Subseq...To investigate the in vitro digestion and fermentation properties of soybean oligosaccharides(SBOS)extracted from defatted soybean meal,the changes in monosaccharide composition and molecular mass were analyzed.Subsequently,the effect of SBOS on microbial community structure and metabolites was studied by 16S rRNA gene sequencing and untargeted metabolomics based on liquid chromatography-mass spectrometry.Results showed that SBOS was not easily enzymolyzed during simulated digestion and could reach the large intestine through the digestive system.The significant decrease in the molecular mass of SBOS after in vitro fermentation indicated its utilization by the gut microbiota,which increased the contents of short-chain fatty acids and lactic acid,thereby reducing the pH of the fermentation broth.Moreover,the core community was found to consist of Blautia,Lactobacillaceae,and Pediococcus.SBOS up-regulated beneficial differential metabolites such as myo-inositol,lactose,and glucose,which were closely related to galactose,amino sugar,and nucleotide sugar metabolism.This study will provide a reference for exploring the relationship between the gut microbiota and the metabolites of SBOS,and provide a basis for the development and application of SBOS as an ingredient for functional products.展开更多
文摘研究辣椒素(capsaicin,CAP)对高糖高脂饮食(high-fructose and high-fat diet,HFFD)喂养小鼠肝脏与胰腺葡萄糖代谢的昼夜节律调节作用。结果表明,CAP缓解了HFFD诱导的小鼠胰岛素抵抗,并增加了肝脏的糖原含量。CAP恢复了肝脏和和胰腺中生物钟基因和葡萄糖代谢相关基因的节律性表达,改善了HFFD小鼠的胰腺功能障碍,并重编程了其粪便代谢物。这些发现表明CAP可能作为一种功能性食品改善葡萄糖代谢的昼夜节律,同时为预防代谢紊乱和昼夜节律失调提供了更有效的饮食干预策略。
文摘To investigate the in vitro digestion and fermentation properties of soybean oligosaccharides(SBOS)extracted from defatted soybean meal,the changes in monosaccharide composition and molecular mass were analyzed.Subsequently,the effect of SBOS on microbial community structure and metabolites was studied by 16S rRNA gene sequencing and untargeted metabolomics based on liquid chromatography-mass spectrometry.Results showed that SBOS was not easily enzymolyzed during simulated digestion and could reach the large intestine through the digestive system.The significant decrease in the molecular mass of SBOS after in vitro fermentation indicated its utilization by the gut microbiota,which increased the contents of short-chain fatty acids and lactic acid,thereby reducing the pH of the fermentation broth.Moreover,the core community was found to consist of Blautia,Lactobacillaceae,and Pediococcus.SBOS up-regulated beneficial differential metabolites such as myo-inositol,lactose,and glucose,which were closely related to galactose,amino sugar,and nucleotide sugar metabolism.This study will provide a reference for exploring the relationship between the gut microbiota and the metabolites of SBOS,and provide a basis for the development and application of SBOS as an ingredient for functional products.
