Metallothionein, a class of low molecular weight, metal- and cysteine-rich Proteins, are postulated to play a central role in metal-related biological processes such as the detoxification of Cd2+and Pb2+. The thermal ...Metallothionein, a class of low molecular weight, metal- and cysteine-rich Proteins, are postulated to play a central role in metal-related biological processes such as the detoxification of Cd2+and Pb2+. The thermal Properties of the interactions of rabbit liver Zn7MT2 and apo-MT2 with Cd2+ and Pb2+ were studied using isothermal titration calorimeter. The same product Cd7MT formed in the reactions of Cd2+ with Zn7MT2 and apo-MT2, while the reactions of Ph2+ with Zn7MT2 and apo-MT2 Produced two MT species Ph7MT and Ph7MT’. The thermodynamic parameters （△H、 △G、 △S, etc. ） for the reactions of MT with 1 mol metal ions were measured at 293. 15 K, pH 4.70. All of these reachons are spontaneous, exothermic processes. The values of △G and K show that Cd2+ is more affinitive to MT than Ph2+. The equilibrium constants K of the replacement reactions of Zn7MT2 by 1 mol Cd2+ and Pb2+ are 2. 7×105 and 1. 3×105 respectively, that means the concentrations of free Cd2+ and Pb2+ are depressed to about 1. 0×10-5 after the reactions with Zn7MT2. These data can be used to evaluate quantitatively the ability of MT in Cd2+ and Ph2+ detoxificatio.展开更多
目的模拟人体生理条件(p H 7.4)下阿托伐他汀(atorvastatin)和人血清白蛋白(HSA)的相互作用的过程。方法采用圆二色谱、荧光光谱、等温滴定量热法和分子对接模拟法,测定结合方式、结合位点数、热力学参数和分子结合距离等相关信息。结果...目的模拟人体生理条件(p H 7.4)下阿托伐他汀(atorvastatin)和人血清白蛋白(HSA)的相互作用的过程。方法采用圆二色谱、荧光光谱、等温滴定量热法和分子对接模拟法,测定结合方式、结合位点数、热力学参数和分子结合距离等相关信息。结果在25℃下,随着atorvastatin逐渐加入到HSA中,α-螺旋含量从58.34%逐渐减少到28.13%(Catorvastatin/CHSA=10.0);atorvastatin以静态淬灭的方式淬灭了HSA的内源性荧光;通过疏水-疏水和氢键作用结合了血清白蛋白且结合比为1∶1;分子对接结果表明,与HSA最有可能的结合位点为214位色氨酸残基附近。结论为阐述他汀类药物的药代动力学行为机制提供科学依据。展开更多
文摘Metallothionein, a class of low molecular weight, metal- and cysteine-rich Proteins, are postulated to play a central role in metal-related biological processes such as the detoxification of Cd2+and Pb2+. The thermal Properties of the interactions of rabbit liver Zn7MT2 and apo-MT2 with Cd2+ and Pb2+ were studied using isothermal titration calorimeter. The same product Cd7MT formed in the reactions of Cd2+ with Zn7MT2 and apo-MT2, while the reactions of Ph2+ with Zn7MT2 and apo-MT2 Produced two MT species Ph7MT and Ph7MT’. The thermodynamic parameters （△H、 △G、 △S, etc. ） for the reactions of MT with 1 mol metal ions were measured at 293. 15 K, pH 4.70. All of these reachons are spontaneous, exothermic processes. The values of △G and K show that Cd2+ is more affinitive to MT than Ph2+. The equilibrium constants K of the replacement reactions of Zn7MT2 by 1 mol Cd2+ and Pb2+ are 2. 7×105 and 1. 3×105 respectively, that means the concentrations of free Cd2+ and Pb2+ are depressed to about 1. 0×10-5 after the reactions with Zn7MT2. These data can be used to evaluate quantitatively the ability of MT in Cd2+ and Ph2+ detoxificatio.
基金supported by the National Natural Science Foundation of China(21203166,21473157)Natural Science Foundation of Zhejiang Province,China(LY16B030001)Food Science and Engineering the Most Important Discipline of Zhejiang Province,China(JYTsp2014111)~~
文摘目的模拟人体生理条件(p H 7.4)下阿托伐他汀(atorvastatin)和人血清白蛋白(HSA)的相互作用的过程。方法采用圆二色谱、荧光光谱、等温滴定量热法和分子对接模拟法,测定结合方式、结合位点数、热力学参数和分子结合距离等相关信息。结果在25℃下,随着atorvastatin逐渐加入到HSA中,α-螺旋含量从58.34%逐渐减少到28.13%(Catorvastatin/CHSA=10.0);atorvastatin以静态淬灭的方式淬灭了HSA的内源性荧光;通过疏水-疏水和氢键作用结合了血清白蛋白且结合比为1∶1;分子对接结果表明,与HSA最有可能的结合位点为214位色氨酸残基附近。结论为阐述他汀类药物的药代动力学行为机制提供科学依据。
基金supported by the National Natural Science Foundation of China(21273061,21573061)Scientific Research Project of Higher Education of Henan Province,China(17A150032)~~
