【目的】评估干酪乳酪杆菌Zhang(Lactobacillus casei Zhang,LCZ)在连续传代培养过程中的遗传稳定性。【方法】在MRS液体培养基中连续培养LCZ 100代,分别在第0、25、50、75、100代取样,分析其菌体形态、碳水化合物利用情况及基因组变化...【目的】评估干酪乳酪杆菌Zhang(Lactobacillus casei Zhang,LCZ)在连续传代培养过程中的遗传稳定性。【方法】在MRS液体培养基中连续培养LCZ 100代,分别在第0、25、50、75、100代取样,分析其菌体形态、碳水化合物利用情况及基因组变化。【结果】LCZ在第0、25、50、75、100代的菌体形态及碳水化合物利用情况无显著差异。基因组大小和GC占比在不同代数与原始基因组相比均无显著差异。SNP突变分析显示,不同代数的LCZ基因组高度保守,突变频率较低。平均核苷酸一致性(ANI)和系统发育树结果表明,不同代数的LCZ与原始基因组亲缘关系接近。不同代数的菌株所注释到的碳水化合物活性酶(CAZymes)无显著差异,表明其碳水化合物利用能力一致。【结论】LCZ在连续传代过程中表现出较高的遗传稳定性。展开更多
The provenance trials of Loblolly Pine(%Pinus taeda)% were planted in different environments of Fujian, Hubei and Zhejiang Provinces in Southern China in 1983. The 19 provenances with the same genetic bases were selec...The provenance trials of Loblolly Pine(%Pinus taeda)% were planted in different environments of Fujian, Hubei and Zhejiang Provinces in Southern China in 1983. The 19 provenances with the same genetic bases were selected to evaluate the effects of planting environments on trees site productivity of Loblolly Pine,its wood properties and their genotypic stabilities with the ecovalence of Wricke’s model and regression coefficient B_i of the Finlay and Wilkinson’s model. The results showed that tree height, DBH, volume, latewood percent, tracheid length and wood basic density of the 19 common provenances of Loblolly Pine for pulpwood were significant affected by the gene effects of these provenances, planting environment, interaction of provenance gene and environment. The regression coefficient and ecovalence for characteristics of its tree growth and wood properties in provenance level were different. Genetic stabilities in tree growth characteristics for these provenances were better, but their genetic stabilities were not good in wood properties. Genetic stabilities were not in the same pattern in characteristics of tree growth and wood properties for provenances. Selection of better provenances should be conducted from tree growth and wood properties on the bases of trial in local districts and these better provenances are not suitable to other districts. The differences among three trial places of Fujiang, Zhejiang and Hubei were up to 12.6%～18.6% in tracheid length, 62%～159% in latewood percent and 4.8%～13.4% in wood basic density. Trees increment and wood properties of Loblolly Pine were mainly affected by planting environments. In order to increase site productivity for industrial wood in afforestation of Loblolly Pine, superior provenance is important and selecting better sites and their reasonable management is also necessary.展开更多
文摘【目的】评估干酪乳酪杆菌Zhang(Lactobacillus casei Zhang,LCZ)在连续传代培养过程中的遗传稳定性。【方法】在MRS液体培养基中连续培养LCZ 100代,分别在第0、25、50、75、100代取样,分析其菌体形态、碳水化合物利用情况及基因组变化。【结果】LCZ在第0、25、50、75、100代的菌体形态及碳水化合物利用情况无显著差异。基因组大小和GC占比在不同代数与原始基因组相比均无显著差异。SNP突变分析显示,不同代数的LCZ基因组高度保守,突变频率较低。平均核苷酸一致性(ANI)和系统发育树结果表明,不同代数的LCZ与原始基因组亲缘关系接近。不同代数的菌株所注释到的碳水化合物活性酶(CAZymes)无显著差异,表明其碳水化合物利用能力一致。【结论】LCZ在连续传代过程中表现出较高的遗传稳定性。
文摘The provenance trials of Loblolly Pine(%Pinus taeda)% were planted in different environments of Fujian, Hubei and Zhejiang Provinces in Southern China in 1983. The 19 provenances with the same genetic bases were selected to evaluate the effects of planting environments on trees site productivity of Loblolly Pine,its wood properties and their genotypic stabilities with the ecovalence of Wricke’s model and regression coefficient B_i of the Finlay and Wilkinson’s model. The results showed that tree height, DBH, volume, latewood percent, tracheid length and wood basic density of the 19 common provenances of Loblolly Pine for pulpwood were significant affected by the gene effects of these provenances, planting environment, interaction of provenance gene and environment. The regression coefficient and ecovalence for characteristics of its tree growth and wood properties in provenance level were different. Genetic stabilities in tree growth characteristics for these provenances were better, but their genetic stabilities were not good in wood properties. Genetic stabilities were not in the same pattern in characteristics of tree growth and wood properties for provenances. Selection of better provenances should be conducted from tree growth and wood properties on the bases of trial in local districts and these better provenances are not suitable to other districts. The differences among three trial places of Fujiang, Zhejiang and Hubei were up to 12.6%～18.6% in tracheid length, 62%～159% in latewood percent and 4.8%～13.4% in wood basic density. Trees increment and wood properties of Loblolly Pine were mainly affected by planting environments. In order to increase site productivity for industrial wood in afforestation of Loblolly Pine, superior provenance is important and selecting better sites and their reasonable management is also necessary.
