Designing a new material to fabricate electrodes sensitive to ion species of specific interest is the main stream in the ion sensitive electrode research. The research efforts of the present authors have been directed...Designing a new material to fabricate electrodes sensitive to ion species of specific interest is the main stream in the ion sensitive electrode research. The research efforts of the present authors have been directed, in particular, to the development of a phosphate ion sensitive electrode based on non-stoichiometric compound Co1-rO and a Cu2+ ion sensitive electrode based on LaB6. In this paper, a new sulfate ion sensitive electrode is reported based on non-crystal Ni-P alloy, which has a very nice response characteristics including stability, selectivity and linear response range. The electrode shows a linear range of response from 1×10-6 to 1 X 10-2 mol/L and a detection limit of 4×10-7mol/L for the sulfate ion. The selectivity coefficients for common anions Cl-, NO3-, H2PO4-, Br- and I- are obtained as 2×10-1, 8×10-5, 8×10-3, 3×10-1 and 4×10-1, respectively. The electrochemical deposition of the non-crystal Ni-P alloy on different matrix materials has been described for preparation of the titled electrode.展开更多
目的:探讨酸敏感离子通道1(acid-sensing ion channel 1,ASIC1)通过细胞外信号调节激酶5(extracellular-signalregulated protein kinase 5,ERK5)信号通路和线粒体紊乱途径介导髓核细胞凋亡的相关机制。方法:自2020年1月至2022年12月收...目的:探讨酸敏感离子通道1(acid-sensing ion channel 1,ASIC1)通过细胞外信号调节激酶5(extracellular-signalregulated protein kinase 5,ERK5)信号通路和线粒体紊乱途径介导髓核细胞凋亡的相关机制。方法:自2020年1月至2022年12月收治34例退行性腰椎间盘突出症患者作为研究对象,男21例,女13例;年龄29~52(37.43±4.75)岁;按照Pfirrmann腰椎间盘退变分级标准,Ⅱ级22例,Ⅳ级12例;L4,515例,L5S119例。取术中获得的LDH患者髓核组织进行免疫组织化学染色,检测ASIC1表达水平。通过原代培养法培养髓核细胞,通过甲苯胺蓝染色和免疫组织化学染色法进行鉴定,免疫荧光染色定位ASIC1蛋白表达。根据是否加入siRNA-ASIC1,ASIC1过表达质粒,以及ERK5的抑制剂对髓核细胞进行分组,即siRNA沉默组、过表达组和抑制剂组,每组3例。干预72 h后收集各组细胞,采用反转录-聚合酶链反应(reverse transcription-polymerase chain reaction,RT-PCR)检测ASIC1,ERK5及B淋巴细胞瘤-2基因相关启动子(BCL-xL/BCL-2-associated death promoter,Bad),B淋巴细胞瘤-2基因相关X蛋白(B-cell lymphoma-2 Associated X,Bax)和抗凋亡因子B淋巴细胞瘤-2(B-cell lymphoma-2,Bcl-2)的表达,钙离子试剂盒检测细胞内钙离子水平,JC-1试剂盒检测细胞线粒体膜电位,AV-PI试剂盒检测细胞的凋亡。结果:Ⅳ级LDH患者术中取出的髓核组织弹性较差,呈白色,延展性差,免疫组织化学结果显示ASIC1表达升高。siRNA沉默组ASIC1的mRNA相对表达量(0.31±0.03)与抑制剂组(0.39±0.05)比较,差异无统计学意义(P>0.05);siRNA沉默组ERK5的mRNA相对表达量(0.32±0.05)与抑制剂组(0.15±0.04)比较,差异有统计学意义(P<0.05),提示ERK5为ASIC1的下游分子。siRNA沉默组和抑制剂组凋亡促进因子Bad,Bax的mRNA相对表达量低于过表达组,差异有统计学意义(P<0.05),抗凋亡因子Bcl-2的mRNA的相对表达量明显升高,差异有统计学意义(P<0.05);过表达组细胞中钙离子含量高于siRNA沉默组和抑制剂组,差异有统计学意义(P<0.05),过表达组细胞中线粒体膜电位正常比例低于siRNA沉默组和抑制剂组,差异有统计学意义(P<0.05),过表达组细胞凋亡率高于siRNA沉默组和抑制剂组,差异有统计学意义(P<0.05)。结论:ASIC1通道蛋白激活后,钙离子进入细胞,作为第二信使分子,通过ERK5信号通路和线粒体紊乱途径,调控髓核细胞的凋亡。展开更多
文摘Designing a new material to fabricate electrodes sensitive to ion species of specific interest is the main stream in the ion sensitive electrode research. The research efforts of the present authors have been directed, in particular, to the development of a phosphate ion sensitive electrode based on non-stoichiometric compound Co1-rO and a Cu2+ ion sensitive electrode based on LaB6. In this paper, a new sulfate ion sensitive electrode is reported based on non-crystal Ni-P alloy, which has a very nice response characteristics including stability, selectivity and linear response range. The electrode shows a linear range of response from 1×10-6 to 1 X 10-2 mol/L and a detection limit of 4×10-7mol/L for the sulfate ion. The selectivity coefficients for common anions Cl-, NO3-, H2PO4-, Br- and I- are obtained as 2×10-1, 8×10-5, 8×10-3, 3×10-1 and 4×10-1, respectively. The electrochemical deposition of the non-crystal Ni-P alloy on different matrix materials has been described for preparation of the titled electrode.
