Electroanalytic direct determination of aluminum is a difficult subject because of the highly negative reductive potential of Al(Ⅲ) and interference of H +. But developing methods for the determination of trace amoun...Electroanalytic direct determination of aluminum is a difficult subject because of the highly negative reductive potential of Al(Ⅲ) and interference of H +. But developing methods for the determination of trace amount of aluminum in environmental and biological samples is a hot topic in recent decades owning to the recognition of the potential toxic effects of aluminum. In this paper, the indirect differential pulse voltammetric(DPV) determination of aluminum with L dopa(3,4 dihydroxyphenylalanine) on glass carbon working electrode has been reported. This novel method bases on the linear decrease of DPV anodic peak current of L dopa with the concentration of aluminum added. Under the optimum experimental conditions (pH 8 5, 0 08 mol/L buffer solution, 4×10 -4 mol/L L dopa), the linear range and the detection limit are (2—18)×10 -7 and 7 6×10 -8 mol/L Al(Ⅲ) respectively, the relative standard deviation for 2×10 -5 mol/L Al (Ⅲ) is 2 4%(n=10). A number of foreign species, especially those necessary components in human body such as ascorbic acid, citrate, phosphate, Fe(Ⅲ), Fe(Ⅱ), Cu(Ⅱ), Zn(Ⅱ), Ca(Ⅱ) and Mg(Ⅱ) for interference have been studied. Satisfactory recoveries have been obtained by applying this method to determining aluminum in biological samples including mineral water, synthetic renal dialysate, urine and hair. The significance of the method and solutions to possible interference encountered in in vivo detection are discussed. A new direction for further development of the in vivo determination of aluminum is provided in this studies.展开更多
A differential pulse voltammetric immunoassay associated with the TMB-H2O2-HRP for the determination of IgE antibody was developed. The enzymatic reaction product produced by HRP catalyzing the oxidation of H2O2 with ...A differential pulse voltammetric immunoassay associated with the TMB-H2O2-HRP for the determination of IgE antibody was developed. The enzymatic reaction product produced by HRP catalyzing the oxidation of H2O2 with TMB exhibits a sensitive voltammet-ric response at 0.10V(vs.Ag/AgCl) in pH4.0 phosphate-citrate buffer solution. The de-tection limit of the present method for IgE antibody was 4.0IU/mL, which was four times lower than that obtained by traditional spectrophotometric ELISA procedure. It has been ap-plied to the determination of IgE in human serum with satisfactory results.展开更多
文摘Electroanalytic direct determination of aluminum is a difficult subject because of the highly negative reductive potential of Al(Ⅲ) and interference of H +. But developing methods for the determination of trace amount of aluminum in environmental and biological samples is a hot topic in recent decades owning to the recognition of the potential toxic effects of aluminum. In this paper, the indirect differential pulse voltammetric(DPV) determination of aluminum with L dopa(3,4 dihydroxyphenylalanine) on glass carbon working electrode has been reported. This novel method bases on the linear decrease of DPV anodic peak current of L dopa with the concentration of aluminum added. Under the optimum experimental conditions (pH 8 5, 0 08 mol/L buffer solution, 4×10 -4 mol/L L dopa), the linear range and the detection limit are (2—18)×10 -7 and 7 6×10 -8 mol/L Al(Ⅲ) respectively, the relative standard deviation for 2×10 -5 mol/L Al (Ⅲ) is 2 4%(n=10). A number of foreign species, especially those necessary components in human body such as ascorbic acid, citrate, phosphate, Fe(Ⅲ), Fe(Ⅱ), Cu(Ⅱ), Zn(Ⅱ), Ca(Ⅱ) and Mg(Ⅱ) for interference have been studied. Satisfactory recoveries have been obtained by applying this method to determining aluminum in biological samples including mineral water, synthetic renal dialysate, urine and hair. The significance of the method and solutions to possible interference encountered in in vivo detection are discussed. A new direction for further development of the in vivo determination of aluminum is provided in this studies.
基金financially supported by Natural Science Foundation of Daton(No.2019168,No.201819)Natural Science Foundation of Shanxi Province(No.201701D121016)research funds of Innovation&Application Engineering Research Center for Mesoporous Materials of Shanxi Province(No.MMIA2019105).
文摘A differential pulse voltammetric immunoassay associated with the TMB-H2O2-HRP for the determination of IgE antibody was developed. The enzymatic reaction product produced by HRP catalyzing the oxidation of H2O2 with TMB exhibits a sensitive voltammet-ric response at 0.10V(vs.Ag/AgCl) in pH4.0 phosphate-citrate buffer solution. The de-tection limit of the present method for IgE antibody was 4.0IU/mL, which was four times lower than that obtained by traditional spectrophotometric ELISA procedure. It has been ap-plied to the determination of IgE in human serum with satisfactory results.
