A processed pseudogene cDNA of PRS1 was first cloned from lymphoblast cells of normal persons and primary gout patients among Chinese. The full length of the pseudogene cDNA was 985 bp, which possessed approx. 89 7% h...A processed pseudogene cDNA of PRS1 was first cloned from lymphoblast cells of normal persons and primary gout patients among Chinese. The full length of the pseudogene cDNA was 985 bp, which possessed approx. 89 7% homology with the published sequence. Except many point mutations, two frame shift deletion of 5 bp and 8 bp were also found at the location 366 bp and 648 bp of the pseudogene cDNA, respectively. All led to 13 stop codons, so that fragment can’t translate. According to these characteristics, the fragment was thought as a processed pseudogene of PRS1.展开更多
采用RT-PCR和RACE(Rapid amplification of c DNA ends)技术,从艾纳香(Blumea balsamifera L·DC)的叶片中克隆到二萜化合物合成的关键酶牻牛儿基牻牛儿基焦磷酸合成酶(Bb GGPS)基因。结果显示:Bb GGPS基因的c DNA全长1475 bp,包含...采用RT-PCR和RACE(Rapid amplification of c DNA ends)技术,从艾纳香(Blumea balsamifera L·DC)的叶片中克隆到二萜化合物合成的关键酶牻牛儿基牻牛儿基焦磷酸合成酶(Bb GGPS)基因。结果显示:Bb GGPS基因的c DNA全长1475 bp,包含开放阅读框(ORF)1002 bp,编码334个氨基酸;亚细胞结构定位于叶绿体,既非膜蛋白也非分泌性蛋白。疏水性分析显示,Bb GGPS是亲水性蛋白。同源性比对结果显示,Bb GGPS蛋白与其他植物中GGPS蛋白具有高度的相似性。系统发育分析表明,所有序列被聚为5大类,Bb GGPS与菊科植物刺菜蓟聚(Cynara cardunculus var)为一类,表明与其亲缘关系最近。展开更多
文摘A processed pseudogene cDNA of PRS1 was first cloned from lymphoblast cells of normal persons and primary gout patients among Chinese. The full length of the pseudogene cDNA was 985 bp, which possessed approx. 89 7% homology with the published sequence. Except many point mutations, two frame shift deletion of 5 bp and 8 bp were also found at the location 366 bp and 648 bp of the pseudogene cDNA, respectively. All led to 13 stop codons, so that fragment can’t translate. According to these characteristics, the fragment was thought as a processed pseudogene of PRS1.
