Skin care products with carbonic acid(H_(2)CO_(3))have gained extensive attention worldwide.However,the conversion of CO_(2) to H_(2)CO_(3) is not stable,and the mechanism of the effect of H_(2)CO_(3) on skin care has...Skin care products with carbonic acid(H_(2)CO_(3))have gained extensive attention worldwide.However,the conversion of CO_(2) to H_(2)CO_(3) is not stable,and the mechanism of the effect of H_(2)CO_(3) on skin care has not been clearly proved.The hydration-dissolution behaviors of CO_(2) were investigated under different temperature,pH,and pressure conditions.Moreover,based on the phenomenon of CO_(2) hydration transformation,the inflammatory effect of CO_(2) hydrate on macrophages(RAW 264.7)was investigated.The result shows that the increase in temperature weakened the hydration of CO_(2),and the increase in pH and pressure both promoted the water-phase transformation of CO_(2).When pH<6,CO_(2) reacts with water to generate H_(2)CO_(3).When pH was between 6-7,the prompt solution was a mixture of H_(2)CO_(3) and HCO_(3)^(-).When the pH was between 7-9,they mainly generated HCO_(3)^(-).And when pH>9,CO_(2) solubility mainly converts to CO_(3)^(2-).Besides,CO_(2) can inhibit the secretion of inflammatory factors by RAW 264.7 cells by inhibiting the phosphorylation of the p38 protein.CO_(2) hydrate inhibited the expression of pro-inflammatory factors IL-6,TNF-α,and up-regulated the expression of anti-inflammatory factor IL-10.Furthermore,the anti-inflammatory molecular mechanism of CO_(2) hydration inhibited the MAPK signaling pathway by inhibiting the phosphorylation of p38.The hydration-dissolution behavior of CO_(2) was investigated.This work revealed the anti-inflammatory bioeffect of CO_(2) hydrate,providing a theoretical basis and application support for CO_(2) skin care products.展开更多
文摘Skin care products with carbonic acid(H_(2)CO_(3))have gained extensive attention worldwide.However,the conversion of CO_(2) to H_(2)CO_(3) is not stable,and the mechanism of the effect of H_(2)CO_(3) on skin care has not been clearly proved.The hydration-dissolution behaviors of CO_(2) were investigated under different temperature,pH,and pressure conditions.Moreover,based on the phenomenon of CO_(2) hydration transformation,the inflammatory effect of CO_(2) hydrate on macrophages(RAW 264.7)was investigated.The result shows that the increase in temperature weakened the hydration of CO_(2),and the increase in pH and pressure both promoted the water-phase transformation of CO_(2).When pH<6,CO_(2) reacts with water to generate H_(2)CO_(3).When pH was between 6-7,the prompt solution was a mixture of H_(2)CO_(3) and HCO_(3)^(-).When the pH was between 7-9,they mainly generated HCO_(3)^(-).And when pH>9,CO_(2) solubility mainly converts to CO_(3)^(2-).Besides,CO_(2) can inhibit the secretion of inflammatory factors by RAW 264.7 cells by inhibiting the phosphorylation of the p38 protein.CO_(2) hydrate inhibited the expression of pro-inflammatory factors IL-6,TNF-α,and up-regulated the expression of anti-inflammatory factor IL-10.Furthermore,the anti-inflammatory molecular mechanism of CO_(2) hydration inhibited the MAPK signaling pathway by inhibiting the phosphorylation of p38.The hydration-dissolution behavior of CO_(2) was investigated.This work revealed the anti-inflammatory bioeffect of CO_(2) hydrate,providing a theoretical basis and application support for CO_(2) skin care products.
