目的:观察姜黄素(curcumin,Cur)水溶性制剂对藻酸盐包被小鼠结肠癌细胞C26在体诱导肿瘤血管生成的抑制作用。方法:以羟丙基-β-环糊精(hydroxypropyl-β-cyclodextrin,HP-β-CD)为包合剂制备姜黄素的水溶性制剂:姜黄素-羟丙基-β-环糊...目的:观察姜黄素(curcumin,Cur)水溶性制剂对藻酸盐包被小鼠结肠癌细胞C26在体诱导肿瘤血管生成的抑制作用。方法:以羟丙基-β-环糊精(hydroxypropyl-β-cyclodextrin,HP-β-CD)为包合剂制备姜黄素的水溶性制剂:姜黄素-羟丙基-β-环糊精包合物(curcumin with hydroxypropyl-β-cyclodextrin,C-HP-β-CD);采用小鼠结肠腺癌C26细胞的藻酸盐包被珠皮下植入制备荷瘤BALB/c鼠动物模型。以C-HP-β-CD腹腔注射荷瘤模型鼠,以烟曲霉素醇(TNF-70)腹腔注射为阳性药物对照,以HP-β-CD注射为阴性对照;采用光学显微镜、荧光显微镜及透射电子显微镜观察藻酸盐包被珠内新生血管的形态;采用异硫氰酸荧光素-葡聚糖(fluorescein isothiacyanate-dextran,FITC-Dextran)示踪技术观测C-HP-β-CD对藻酸盐包被C26细胞诱发血管生成的抑制作用。结果:成功制备C-HP-β-CD和C26细胞藻酸盐包被珠鼠荷瘤模型,透射电子显微镜观察包被珠内的肿瘤细胞团中可见血管腔形成,部分管腔的周围含有棒状体的内皮细胞。光学显微镜及荧光显微镜观察表明,姜黄素组及阳性对照组的包被珠内新生血管密度明显低于阴性对照组,但3组包被珠内的新生血管形态无明显区别;荧光定量分析表明,姜黄素组及阳性对照组包被珠的荧光强度显著低于阴性对照组(P<0.01),表明前两者的血管生成受到明显抑制,但姜黄素组与阳性对照组之间相差不明显。结论:采用环糊精包合法制备的姜黄素水溶性制剂便于注射给药,其抗肿瘤新生血管作用明显,可望开发成为一种新型的肿瘤血管生成抑制剂。展开更多
Magnetic starch particles （MSPs） were synthesized in water-in-oil mieroemulsion at room temperature. MSPs were characterized by transmission electron microscopy （TEM）, Fourier transform infrared spectrometry （FTI...Magnetic starch particles （MSPs） were synthesized in water-in-oil mieroemulsion at room temperature. MSPs were characterized by transmission electron microscopy （TEM）, Fourier transform infrared spectrometry （FTIR）, zeta potential system, thermogravimetric analysis （TGA） and vibrating sample magnetometry （VSM）. The average diameter of the MSPs was 220 nm, dispersed with well-proportioned size and magnetic resonance, the saturation magnetization was 3.64 A.mR/kg. MSP was coated with poly-L-lysine （PLL）, and then the surface of PLL-MSP was combined with fluorescein isothiocynate （FITC）. Results show that fluorescent/magnetic starch particles （FMSPs） are of stable photo-bleaching capability compared with free FITC, with low bio-toxicity and certain function of magnetic separation. It is expected that FMSPs are bifimctional nano-materials including fluorescence labelling and magnetic separation.展开更多
文摘目的:观察姜黄素(curcumin,Cur)水溶性制剂对藻酸盐包被小鼠结肠癌细胞C26在体诱导肿瘤血管生成的抑制作用。方法:以羟丙基-β-环糊精(hydroxypropyl-β-cyclodextrin,HP-β-CD)为包合剂制备姜黄素的水溶性制剂:姜黄素-羟丙基-β-环糊精包合物(curcumin with hydroxypropyl-β-cyclodextrin,C-HP-β-CD);采用小鼠结肠腺癌C26细胞的藻酸盐包被珠皮下植入制备荷瘤BALB/c鼠动物模型。以C-HP-β-CD腹腔注射荷瘤模型鼠,以烟曲霉素醇(TNF-70)腹腔注射为阳性药物对照,以HP-β-CD注射为阴性对照;采用光学显微镜、荧光显微镜及透射电子显微镜观察藻酸盐包被珠内新生血管的形态;采用异硫氰酸荧光素-葡聚糖(fluorescein isothiacyanate-dextran,FITC-Dextran)示踪技术观测C-HP-β-CD对藻酸盐包被C26细胞诱发血管生成的抑制作用。结果:成功制备C-HP-β-CD和C26细胞藻酸盐包被珠鼠荷瘤模型,透射电子显微镜观察包被珠内的肿瘤细胞团中可见血管腔形成,部分管腔的周围含有棒状体的内皮细胞。光学显微镜及荧光显微镜观察表明,姜黄素组及阳性对照组的包被珠内新生血管密度明显低于阴性对照组,但3组包被珠内的新生血管形态无明显区别;荧光定量分析表明,姜黄素组及阳性对照组包被珠的荧光强度显著低于阴性对照组(P<0.01),表明前两者的血管生成受到明显抑制,但姜黄素组与阳性对照组之间相差不明显。结论:采用环糊精包合法制备的姜黄素水溶性制剂便于注射给药,其抗肿瘤新生血管作用明显,可望开发成为一种新型的肿瘤血管生成抑制剂。
基金Project(200501) supported by the "985" Program of China
文摘Magnetic starch particles （MSPs） were synthesized in water-in-oil mieroemulsion at room temperature. MSPs were characterized by transmission electron microscopy （TEM）, Fourier transform infrared spectrometry （FTIR）, zeta potential system, thermogravimetric analysis （TGA） and vibrating sample magnetometry （VSM）. The average diameter of the MSPs was 220 nm, dispersed with well-proportioned size and magnetic resonance, the saturation magnetization was 3.64 A.mR/kg. MSP was coated with poly-L-lysine （PLL）, and then the surface of PLL-MSP was combined with fluorescein isothiocynate （FITC）. Results show that fluorescent/magnetic starch particles （FMSPs） are of stable photo-bleaching capability compared with free FITC, with low bio-toxicity and certain function of magnetic separation. It is expected that FMSPs are bifimctional nano-materials including fluorescence labelling and magnetic separation.
