p-t-Butyl-calixarene bonded silica stationary phase(CBS) for high performance liquid chromatography was prepared via condensation reaction on the surface of solid .The stationary phase was characterized by means of el...p-t-Butyl-calixarene bonded silica stationary phase(CBS) for high performance liquid chromatography was prepared via condensation reaction on the surface of solid .The stationary phase was characterized by means of elemental analysis and FTIR. The chromatographic performance of CBS was studied by using disubstituted benzenes as the solutes and methanol-water or methanol-buffer as the mobile phases, and compared with that obtained on CPS and ODS. The results show that the chromrtographic process can proceed in various retention mechanism, such as π-π, hydrogen-bonding, charge-transfer and inclusion complexation, besides hydrophobic interaction.展开更多
A novel chiral bonded stationary phase(CBSP) for ligand exchange chromatography was prepared by bonding ( S ) 1,2,3,4 tetrahydro 3 isoquinoline carboxylic acid prepared from L Phe to YWG 80 silica gel via 3 glycidylox...A novel chiral bonded stationary phase(CBSP) for ligand exchange chromatography was prepared by bonding ( S ) 1,2,3,4 tetrahydro 3 isoquinoline carboxylic acid prepared from L Phe to YWG 80 silica gel via 3 glycidyloxypropyltrimethoxysilane as a coupling agent. Chromatographic resolutions of some DL amino acids were achieved on the CBSP by using an aqueous solution of 2 mmol/L N(C 2H 5) 3, 2 mmol/L HAc and 0 2 mmol/L Cu(Ac) 2 as the mobile phase with a flow rate 1 0 mL/min, column temperature 50 ℃ and detection at 254 nm. The enantioselectivity α of the DL amino acids on the CBSP was found to be between 1 11 and 1 51. The elution order of D isomer before L isomer on the CBSP was observed for all the DL amino acids resolved except DL Val. For DL Pro, DL Val and DL Leu the elution order through the CBSP was different from that through the chiral ligand exchange phases prepared from L Pro or L hydroxyl proline with a five\|membered ring structure. [WT5HZ]展开更多
文摘p-t-Butyl-calixarene bonded silica stationary phase(CBS) for high performance liquid chromatography was prepared via condensation reaction on the surface of solid .The stationary phase was characterized by means of elemental analysis and FTIR. The chromatographic performance of CBS was studied by using disubstituted benzenes as the solutes and methanol-water or methanol-buffer as the mobile phases, and compared with that obtained on CPS and ODS. The results show that the chromrtographic process can proceed in various retention mechanism, such as π-π, hydrogen-bonding, charge-transfer and inclusion complexation, besides hydrophobic interaction.
文摘A novel chiral bonded stationary phase(CBSP) for ligand exchange chromatography was prepared by bonding ( S ) 1,2,3,4 tetrahydro 3 isoquinoline carboxylic acid prepared from L Phe to YWG 80 silica gel via 3 glycidyloxypropyltrimethoxysilane as a coupling agent. Chromatographic resolutions of some DL amino acids were achieved on the CBSP by using an aqueous solution of 2 mmol/L N(C 2H 5) 3, 2 mmol/L HAc and 0 2 mmol/L Cu(Ac) 2 as the mobile phase with a flow rate 1 0 mL/min, column temperature 50 ℃ and detection at 254 nm. The enantioselectivity α of the DL amino acids on the CBSP was found to be between 1 11 and 1 51. The elution order of D isomer before L isomer on the CBSP was observed for all the DL amino acids resolved except DL Val. For DL Pro, DL Val and DL Leu the elution order through the CBSP was different from that through the chiral ligand exchange phases prepared from L Pro or L hydroxyl proline with a five\|membered ring structure. [WT5HZ]
