Four methods were used to prepare four crude elicitors containing different ingredients from mycelia of fungus F 5: (a) Polysaccharides A containing lipids and proteins; (b) Micromolecular polysaccharides B without li...Four methods were used to prepare four crude elicitors containing different ingredients from mycelia of fungus F 5: (a) Polysaccharides A containing lipids and proteins; (b) Micromolecular polysaccharides B without lipids and proteins; (c) Macromolecular polysaccharides F without lipids and proteins; (d) Polysaccharides H containing proteins but no lipids. The crude preparation B had the highest ability to induce taxol biosynthesis among other preparations did. The crude preparation B was through Sephadex G15 column to separate two compositions: BⅠ whose molecular weight was over 1500 D and BⅡ whose molecular weight between 700～ 1500 D. The BⅡ activity to induce taxol biosynthesis was 2.3 times higher than that of the crude preparation B, while BⅠ hadn’t the activity to induce taxol biosynthesis. By analysis of PAL activity induced by elicitors, we conclude that the changes of PAL activity could be regarded as a useful physiological indicator to select the effective elicitor.展开更多
考察了6种真菌诱导物对高山红景天(Rhodiola sachalinensis A. Bor)细胞生长与红景天甙积累的影响。其中以黑曲霉诱导物效果最好。在细胞培养初期添加浓度为10mg(carbohydrate)/L的黑曲霉诱导物能使培养细胞中红景天甙含量提高到0.995...考察了6种真菌诱导物对高山红景天(Rhodiola sachalinensis A. Bor)细胞生长与红景天甙积累的影响。其中以黑曲霉诱导物效果最好。在细胞培养初期添加浓度为10mg(carbohydrate)/L的黑曲霉诱导物能使培养细胞中红景天甙含量提高到0.995％。前体与诱导物调控组合运用最终使红景天甙产量达到167.4mg/L,是对照培养的3.5倍。另外,对真菌诱导物的作用机理也进行了探讨,真菌诱导物添加促进红景天甙的积累应该与激活培养细胞中的苯丙烷类代谢途径有关。展开更多
文摘Four methods were used to prepare four crude elicitors containing different ingredients from mycelia of fungus F 5: (a) Polysaccharides A containing lipids and proteins; (b) Micromolecular polysaccharides B without lipids and proteins; (c) Macromolecular polysaccharides F without lipids and proteins; (d) Polysaccharides H containing proteins but no lipids. The crude preparation B had the highest ability to induce taxol biosynthesis among other preparations did. The crude preparation B was through Sephadex G15 column to separate two compositions: BⅠ whose molecular weight was over 1500 D and BⅡ whose molecular weight between 700～ 1500 D. The BⅡ activity to induce taxol biosynthesis was 2.3 times higher than that of the crude preparation B, while BⅠ hadn’t the activity to induce taxol biosynthesis. By analysis of PAL activity induced by elicitors, we conclude that the changes of PAL activity could be regarded as a useful physiological indicator to select the effective elicitor.
文摘考察了6种真菌诱导物对高山红景天(Rhodiola sachalinensis A. Bor)细胞生长与红景天甙积累的影响。其中以黑曲霉诱导物效果最好。在细胞培养初期添加浓度为10mg(carbohydrate)/L的黑曲霉诱导物能使培养细胞中红景天甙含量提高到0.995％。前体与诱导物调控组合运用最终使红景天甙产量达到167.4mg/L,是对照培养的3.5倍。另外,对真菌诱导物的作用机理也进行了探讨,真菌诱导物添加促进红景天甙的积累应该与激活培养细胞中的苯丙烷类代谢途径有关。
