以高通量测序技术Illumina Hi SeqTM2000对稀杯盔形珊瑚(Galaxea astreata)进行转录组测序分析,共获得50 360 620条短序列(reads)。利用Trinity软件对所有reads从头组装后得到81 014条单基因簇(Unigenes),60 471条(74.58%)编码蛋白框(Co...以高通量测序技术Illumina Hi SeqTM2000对稀杯盔形珊瑚(Galaxea astreata)进行转录组测序分析,共获得50 360 620条短序列(reads)。利用Trinity软件对所有reads从头组装后得到81 014条单基因簇(Unigenes),60 471条(74.58%)编码蛋白框(Coding Sequences,CDs)。与Nr(Non-redundant,非冗余)、COG(Cluster of Orthologous Groups of proteins,蛋白相邻类的聚簇)、KEGG(Kyoto encyclopedia of genes and genomes,京都基因与基因组百科全书)、Swissprot四大数据库比对共获得36 545条注释基因。其中与COG数据库比对获得14 491条注释基因,分为24个功能类别,其中参与一般功能预测类的Unigene数最多,有4 642条;KEGG分析比对获得16 021条注释基因,分成241类,包括代谢通路、钙离子信号通路、MAPK信号通路等,在所有通路中参与代谢途径的基因数最多,共有2 450条(15.29%);GO(Gene Ontology,基因本体)功能分类将Unigene分为47个类别。展开更多
为探究不同泡菜水和发酵方式对小米辣品质特性的影响,本研究对小米辣进行新泡菜水自然发酵、新泡菜水混菌(植物乳杆菌Lactiplantibacillus plantarum KUST-D1303:盔形毕赤酵母Pichia manshurica KUST-F1705=1:1)接种发酵、老泡菜水自然...为探究不同泡菜水和发酵方式对小米辣品质特性的影响,本研究对小米辣进行新泡菜水自然发酵、新泡菜水混菌(植物乳杆菌Lactiplantibacillus plantarum KUST-D1303:盔形毕赤酵母Pichia manshurica KUST-F1705=1:1)接种发酵、老泡菜水自然发酵和老泡菜水混菌接种发酵4种方式发酵,并对发酵小米辣的品质特性进行比较分析。结果表明,与其他发酵方式相比,老泡菜水混菌接种发酵显著(P<0.05)增加了发酵小米辣中乳酸菌数量、有机酸含量以及挥发性风味物质,特别是醇类、萜烯类和含硫化合物的种类和含量,降低了亚硝酸盐含量。此外,老泡菜水混菌接种发酵小米辣的pH下降迅速并保持稳定,可延缓小米辣在发酵过程中变黄、变软。综上,老泡菜水混合接种发酵可提高发酵小米辣的安全性、风味组分以及改善产品的滋味,是发酵小米辣的优选工艺。展开更多
The early Silurian Chongqing Lagerstätte(middle Telychian)yields exceptionally preserved articulated jawless and jawed fishes.Here,we describe a new eugaleaspiform(Galeaspida,jawless stem-Gnathostomata),Miaojiaas...The early Silurian Chongqing Lagerstätte(middle Telychian)yields exceptionally preserved articulated jawless and jawed fishes.Here,we describe a new eugaleaspiform(Galeaspida,jawless stem-Gnathostomata),Miaojiaaspis dichotomus gen.et sp.nov.,from the Chongqing Lagerstätte in Xiushan,Chongqing,China.The new form resembles Tujiaaspis vividus in the short medial dorsal canal,and the presence of the branching ends of the lateral transverse canal.They differ in that T.vividus has highly developed subordinate branches of the sensory canals that form a reticulate sensory canal system,and the median dorsal opening is more elongated.Our phylogenetic analysis recovers M.dichotomus and T.vividus as a monophyletic clade(Tujiaaspidae fam.nov.),which is supported by two synapomorphies:the short medial dorsal canal,and the branching ends of the lateral transverse canal.Tujiaaspidae forms a trichotomy with Shuyuidae and a clade comprising Anjiaspis,Sinogaleaspidae,Yongdongaspidae,and the‘eugaleaspid cluster’.The sensory canal patterns in galeaspids are compared to show the transformation sequence of the sensory canal system in Eugaleaspiformes.展开更多
文摘以高通量测序技术Illumina Hi SeqTM2000对稀杯盔形珊瑚(Galaxea astreata)进行转录组测序分析,共获得50 360 620条短序列(reads)。利用Trinity软件对所有reads从头组装后得到81 014条单基因簇(Unigenes),60 471条(74.58%)编码蛋白框(Coding Sequences,CDs)。与Nr(Non-redundant,非冗余)、COG(Cluster of Orthologous Groups of proteins,蛋白相邻类的聚簇)、KEGG(Kyoto encyclopedia of genes and genomes,京都基因与基因组百科全书)、Swissprot四大数据库比对共获得36 545条注释基因。其中与COG数据库比对获得14 491条注释基因,分为24个功能类别,其中参与一般功能预测类的Unigene数最多,有4 642条;KEGG分析比对获得16 021条注释基因,分成241类,包括代谢通路、钙离子信号通路、MAPK信号通路等,在所有通路中参与代谢途径的基因数最多,共有2 450条(15.29%);GO(Gene Ontology,基因本体)功能分类将Unigene分为47个类别。
文摘为探究不同泡菜水和发酵方式对小米辣品质特性的影响,本研究对小米辣进行新泡菜水自然发酵、新泡菜水混菌(植物乳杆菌Lactiplantibacillus plantarum KUST-D1303:盔形毕赤酵母Pichia manshurica KUST-F1705=1:1)接种发酵、老泡菜水自然发酵和老泡菜水混菌接种发酵4种方式发酵,并对发酵小米辣的品质特性进行比较分析。结果表明,与其他发酵方式相比,老泡菜水混菌接种发酵显著(P<0.05)增加了发酵小米辣中乳酸菌数量、有机酸含量以及挥发性风味物质,特别是醇类、萜烯类和含硫化合物的种类和含量,降低了亚硝酸盐含量。此外,老泡菜水混菌接种发酵小米辣的pH下降迅速并保持稳定,可延缓小米辣在发酵过程中变黄、变软。综上,老泡菜水混合接种发酵可提高发酵小米辣的安全性、风味组分以及改善产品的滋味,是发酵小米辣的优选工艺。
文摘The early Silurian Chongqing Lagerstätte(middle Telychian)yields exceptionally preserved articulated jawless and jawed fishes.Here,we describe a new eugaleaspiform(Galeaspida,jawless stem-Gnathostomata),Miaojiaaspis dichotomus gen.et sp.nov.,from the Chongqing Lagerstätte in Xiushan,Chongqing,China.The new form resembles Tujiaaspis vividus in the short medial dorsal canal,and the presence of the branching ends of the lateral transverse canal.They differ in that T.vividus has highly developed subordinate branches of the sensory canals that form a reticulate sensory canal system,and the median dorsal opening is more elongated.Our phylogenetic analysis recovers M.dichotomus and T.vividus as a monophyletic clade(Tujiaaspidae fam.nov.),which is supported by two synapomorphies:the short medial dorsal canal,and the branching ends of the lateral transverse canal.Tujiaaspidae forms a trichotomy with Shuyuidae and a clade comprising Anjiaspis,Sinogaleaspidae,Yongdongaspidae,and the‘eugaleaspid cluster’.The sensory canal patterns in galeaspids are compared to show the transformation sequence of the sensory canal system in Eugaleaspiformes.
