目的从健康妇女阴道分泌物中分离并筛选出对阴道加德纳菌(Gv)或白色假丝酵母菌(Ca)具有抑菌活性的乳酸杆菌,并观察其功能。方法采集30例健康育龄妇女的阴道分泌物,经数次增菌培养-平板分离后选出疑似乳酸杆菌菌株,采用16S r DNA基因测...目的从健康妇女阴道分泌物中分离并筛选出对阴道加德纳菌(Gv)或白色假丝酵母菌(Ca)具有抑菌活性的乳酸杆菌,并观察其功能。方法采集30例健康育龄妇女的阴道分泌物,经数次增菌培养-平板分离后选出疑似乳酸杆菌菌株,采用16S r DNA基因测序法确定菌株类型。采用双层琼脂扩散法评价其中的乳酸杆菌菌株对指示菌(10种细菌和5种假丝酵母菌)的抑菌效果,筛选出具有抑Gv/Ca活性的人阴道乳酸杆菌。采用共孵育实验定量观察筛选出的人阴道乳酸杆菌对Gv/Ca的抑菌活性,同时观察筛选出的乳酸杆菌菌株的产过氧化氢、产乳酸能力,以及自聚合/共聚合能力和生物膜形成能力。结果 30例健康女性阴道分泌物中分离出51株菌,其中23株具有乳酸杆菌特征。16S r DNA基因测序确定其均为乳酸杆菌。双层琼脂扩散实验结果显示,共有8个菌株对Gv/Ca有抑菌作用:包括仅抑制Gv的2号菌株、仅抑制Ca的7、26和44号菌株以及对同时抑制二者的16、19、31和41号菌株。上述8个菌株即为筛选出的有抑Gv/Ca菌活性的人阴道乳酸杆菌。筛选出的8种乳酸杆菌与Gv共培养时,全部菌株均显示了抑菌活性,其中41号菌株完全抑制了Gv的生长;当用乳酸杆菌培养上清液孵育Gv时,全部菌株培养上清液均显示了明显的抑菌活性。当乳酸杆菌与Ca共培养时,全部菌株均显示了抑菌活性;当乳酸杆菌培养上清液孵育Ca时,全部菌株培养上清液均显示了抑菌活性,其中19号菌株完全抑制了Ca的生长。8个乳酸杆菌菌株p H值、乳酸生成量和过氧化氢生成量与阴性对照菌株相比,P均<0.05。其中以19号和41号菌株的产酸和产过氧化氢能力最强,达到了阳性对照菌株水平(P均>0.05)。Gv的自聚合水平为45.25%,而Ca的自聚合水平则高达100%。乳酸杆菌菌株16号(92.57%)、19号(96.32%)、31号(92.16%)和41号(93.52%)均显示了明显的自聚合能力。19号、26号和41号菌株显示了与Ca较强的共聚合能力(定性均为++,定量分别为61.23%、58.72%和54.59%),其他菌株与Ca的共聚合水平为28.53%~44.21%。16号、19号和41号菌株显示了与Gv较强的共聚合能力(定性均为++,定量分别为82.34%、83.41%和93.56%),其他菌株与Ca的共聚合水平为41.33%~46.12%。筛选出的8种乳酸杆菌均能够形成生物膜,其中19号菌株最强,其次是41号菌株。结论成功筛选出8个有抑Gv/Ca活性的人阴道乳酸杆菌菌株。8个菌株的乳酸杆菌均对Gv/Ca有抑菌作用,且有较强的产过氧化氢、乳酸和形成生物膜的能力。其中19、41号菌株具有成为新的阴道益生菌的潜力。展开更多
This paper presented the effects of systemic fluconazole therapy via intravenous (IV) and oral (PO) administrations on the adhesion of Candida albicans (C. albicans) to the buccal epithelial cells (BEC) from five trea...This paper presented the effects of systemic fluconazole therapy via intravenous (IV) and oral (PO) administrations on the adhesion of Candida albicans (C. albicans) to the buccal epithelial cells (BEC) from five treated patients with three candidosis, one mucormycosis and one sporotrichosis and at the same time.an analysis of the cell surface proteins involving candidal adherent receptor in the BEC of the patients in the course of 7 days were exposed to ￣3H-leucine radiolabeled C. albicans for in vitro candidal adherent assay.and the BEC from first intake day and the last intake day of the patients were extracted by dithiothreitol (DTT)-iodoacetamide treatment for SDSPAGE. These results indicate that the systemic fluconazole therapy results in the inhibitory effect of candidal adhesion to BEC of treated patients to prevent them from oral candidosis for a prolonged time, which is based on the absent surface protein (35 KDa) of the BEC.展开更多
Increasing reports on application and safety of liposomal amphotericin B (Amphotec) in the treatment of deep fungal infections have been described recently. This is the first report that a case of liver abscess due to...Increasing reports on application and safety of liposomal amphotericin B (Amphotec) in the treatment of deep fungal infections have been described recently. This is the first report that a case of liver abscess due to Candida albicans was completely cured with intra-abscess and intravenous administration of liposomal amphotericin B without recurrence in three-year follow-up period.展开更多
文摘目的从健康妇女阴道分泌物中分离并筛选出对阴道加德纳菌(Gv)或白色假丝酵母菌(Ca)具有抑菌活性的乳酸杆菌,并观察其功能。方法采集30例健康育龄妇女的阴道分泌物,经数次增菌培养-平板分离后选出疑似乳酸杆菌菌株,采用16S r DNA基因测序法确定菌株类型。采用双层琼脂扩散法评价其中的乳酸杆菌菌株对指示菌(10种细菌和5种假丝酵母菌)的抑菌效果,筛选出具有抑Gv/Ca活性的人阴道乳酸杆菌。采用共孵育实验定量观察筛选出的人阴道乳酸杆菌对Gv/Ca的抑菌活性,同时观察筛选出的乳酸杆菌菌株的产过氧化氢、产乳酸能力,以及自聚合/共聚合能力和生物膜形成能力。结果 30例健康女性阴道分泌物中分离出51株菌,其中23株具有乳酸杆菌特征。16S r DNA基因测序确定其均为乳酸杆菌。双层琼脂扩散实验结果显示,共有8个菌株对Gv/Ca有抑菌作用:包括仅抑制Gv的2号菌株、仅抑制Ca的7、26和44号菌株以及对同时抑制二者的16、19、31和41号菌株。上述8个菌株即为筛选出的有抑Gv/Ca菌活性的人阴道乳酸杆菌。筛选出的8种乳酸杆菌与Gv共培养时,全部菌株均显示了抑菌活性,其中41号菌株完全抑制了Gv的生长;当用乳酸杆菌培养上清液孵育Gv时,全部菌株培养上清液均显示了明显的抑菌活性。当乳酸杆菌与Ca共培养时,全部菌株均显示了抑菌活性;当乳酸杆菌培养上清液孵育Ca时,全部菌株培养上清液均显示了抑菌活性,其中19号菌株完全抑制了Ca的生长。8个乳酸杆菌菌株p H值、乳酸生成量和过氧化氢生成量与阴性对照菌株相比,P均<0.05。其中以19号和41号菌株的产酸和产过氧化氢能力最强,达到了阳性对照菌株水平(P均>0.05)。Gv的自聚合水平为45.25%,而Ca的自聚合水平则高达100%。乳酸杆菌菌株16号(92.57%)、19号(96.32%)、31号(92.16%)和41号(93.52%)均显示了明显的自聚合能力。19号、26号和41号菌株显示了与Ca较强的共聚合能力(定性均为++,定量分别为61.23%、58.72%和54.59%),其他菌株与Ca的共聚合水平为28.53%~44.21%。16号、19号和41号菌株显示了与Gv较强的共聚合能力(定性均为++,定量分别为82.34%、83.41%和93.56%),其他菌株与Ca的共聚合水平为41.33%~46.12%。筛选出的8种乳酸杆菌均能够形成生物膜,其中19号菌株最强,其次是41号菌株。结论成功筛选出8个有抑Gv/Ca活性的人阴道乳酸杆菌菌株。8个菌株的乳酸杆菌均对Gv/Ca有抑菌作用,且有较强的产过氧化氢、乳酸和形成生物膜的能力。其中19、41号菌株具有成为新的阴道益生菌的潜力。
文摘This paper presented the effects of systemic fluconazole therapy via intravenous (IV) and oral (PO) administrations on the adhesion of Candida albicans (C. albicans) to the buccal epithelial cells (BEC) from five treated patients with three candidosis, one mucormycosis and one sporotrichosis and at the same time.an analysis of the cell surface proteins involving candidal adherent receptor in the BEC of the patients in the course of 7 days were exposed to ￣3H-leucine radiolabeled C. albicans for in vitro candidal adherent assay.and the BEC from first intake day and the last intake day of the patients were extracted by dithiothreitol (DTT)-iodoacetamide treatment for SDSPAGE. These results indicate that the systemic fluconazole therapy results in the inhibitory effect of candidal adhesion to BEC of treated patients to prevent them from oral candidosis for a prolonged time, which is based on the absent surface protein (35 KDa) of the BEC.
文摘Increasing reports on application and safety of liposomal amphotericin B (Amphotec) in the treatment of deep fungal infections have been described recently. This is the first report that a case of liver abscess due to Candida albicans was completely cured with intra-abscess and intravenous administration of liposomal amphotericin B without recurrence in three-year follow-up period.
