对在黑龙江哈尔滨闫家岗农场采集的绥农14大豆细菌性斑点病叶片进行病原菌分离,将分离得到的大豆细菌性斑点病病原菌进行抗生素耐性鉴定、分子鉴定并对大豆品种回接试验,分离得到1个细菌菌株。经过革兰氏染色、病原菌回接试验、16S r DN...对在黑龙江哈尔滨闫家岗农场采集的绥农14大豆细菌性斑点病叶片进行病原菌分离,将分离得到的大豆细菌性斑点病病原菌进行抗生素耐性鉴定、分子鉴定并对大豆品种回接试验,分离得到1个细菌菌株。经过革兰氏染色、病原菌回接试验、16S r DNA分子鉴定方法确定分离的菌株为丁香假单胞杆菌属,命名为Psgneau 2。分离的菌株对终浓度为25,50,100μg·m L^(-1)的氨苄青霉素和羧苄青霉素都具有耐性,对壮观青霉素、利福平、卡那霉素、庆大霉素、四环素和氯霉素不具有抗性。采用高压喷雾和菌落滴度的方法将分离的Psgneau 2菌株接种10个大豆品种,进行致病性鉴定,结果表明绥农14极易感病,合丰35抗病性高于其它品种。展开更多
The disinfected bacteria will be a photoreactivation under the irradiation of the sunlight,and the light intensity plays an important role in the bacteria resurrection.The effect of light intensity on photoreactivatio...The disinfected bacteria will be a photoreactivation under the irradiation of the sunlight,and the light intensity plays an important role in the bacteria resurrection.The effect of light intensity on photoreactivation of Escherichia coli(E.coli) and Enterococcus faecalis(E.faecalis) in secondary effluents which were disinfected respectively by pure UV and UV-TiO_2 was investigated.The results show that the disinfection efficiency of UV-TiO_2 is much higher than that of the pure UV disinfection.The photoreactivation rate of E.coli is much higher in pure UV disinfection than in UV-TiO_2 photocatalytic disinfection.Under high light intensity in UV-TiO_2 disinfection,high resurrection rate can be induced.However,a higher resurrection rate can be introduced even under low light intensity in pure UV disinfection alone.Meanwhile,UV-TiO_2 disinfection has a strong inhibition effect on E.faecalis photoreactivation.When the light intensity is lower than 21 μW/cm^2,nearly no resurrection of E.faecalis occurs after 72 h resurrection irradiation,and a little resurrection rate is observed only under a strong photoreactivating light intensity.展开更多
基金Projects(51174090,51168026)supported by the National Natural Science Foundation of China
文摘The disinfected bacteria will be a photoreactivation under the irradiation of the sunlight,and the light intensity plays an important role in the bacteria resurrection.The effect of light intensity on photoreactivation of Escherichia coli(E.coli) and Enterococcus faecalis(E.faecalis) in secondary effluents which were disinfected respectively by pure UV and UV-TiO_2 was investigated.The results show that the disinfection efficiency of UV-TiO_2 is much higher than that of the pure UV disinfection.The photoreactivation rate of E.coli is much higher in pure UV disinfection than in UV-TiO_2 photocatalytic disinfection.Under high light intensity in UV-TiO_2 disinfection,high resurrection rate can be induced.However,a higher resurrection rate can be introduced even under low light intensity in pure UV disinfection alone.Meanwhile,UV-TiO_2 disinfection has a strong inhibition effect on E.faecalis photoreactivation.When the light intensity is lower than 21 μW/cm^2,nearly no resurrection of E.faecalis occurs after 72 h resurrection irradiation,and a little resurrection rate is observed only under a strong photoreactivating light intensity.
