Noncovalent inclusion complex between β-cyclodextrin and sodium houttuyfonate were studied by electrospray ionization mass spectrometry (ESI-MS) and capillary electrophoresis (CE). It was observed that β-cyclodextri...Noncovalent inclusion complex between β-cyclodextrin and sodium houttuyfonate were studied by electrospray ionization mass spectrometry (ESI-MS) and capillary electrophoresis (CE). It was observed that β-cyclodextrin and sodium houttuyfonate integrated at a radio of 1 : 1 by ESI-MS in the negative-ion mode. The relative intensity of the inclusion complex ion was 18% compared with β-cyclodextrin ion, and 20% compared with houttuyfonate ion, respectively. The results indicated that the inclusion complex was considerable steady in the gas phase. The mobilities of houttuyfonate were measured in solution with different concentration of β-cyclodextrin by capillary electrophoresis, and the logarithm of inclusion constant obtained from mobilities were 3. 845, indicated that the interaction of the houttuyfonate and β-cyclodextrin is strong.展开更多
文摘Noncovalent inclusion complex between β-cyclodextrin and sodium houttuyfonate were studied by electrospray ionization mass spectrometry (ESI-MS) and capillary electrophoresis (CE). It was observed that β-cyclodextrin and sodium houttuyfonate integrated at a radio of 1 : 1 by ESI-MS in the negative-ion mode. The relative intensity of the inclusion complex ion was 18% compared with β-cyclodextrin ion, and 20% compared with houttuyfonate ion, respectively. The results indicated that the inclusion complex was considerable steady in the gas phase. The mobilities of houttuyfonate were measured in solution with different concentration of β-cyclodextrin by capillary electrophoresis, and the logarithm of inclusion constant obtained from mobilities were 3. 845, indicated that the interaction of the houttuyfonate and β-cyclodextrin is strong.
文摘建立了高效液相色谱-电喷雾串联质谱(HPLC-ESI MS/MS)分析环境水样中22种抗生素类药物的方法。采用HLB固相萃取柱对环境水样中的目标化合物进行富集、净化,然后以6mL氨水-甲醇(5∶95,v/v)溶液洗脱。收集的洗脱液经氮气吹干至1mL,然后进行HPLC-ESI MS/MS分离分析。色谱流动相A相为甲醇-乙腈(1∶1,v/v),B相为0.3%(体积分数)甲酸水溶液(含0.1%(体积分数)甲酸铵,pH2.9);色谱柱为XTerra MS C18柱。质谱检测采用正离子扫描,多反应监测模式。分别以自来水和污水作为基质,22种抗生素类药物的加标平均回收率分别为54.9%～130%和57.4%～138%,相对标准偏差(n=3)分别为2.85%～28.6%和2.02%～23.2%;方法的检出限为0.05～0.5ng/L。将建立的方法应用于北京市高碑店湖和小清河水样的分析,结果表明在两个水样中均有部分抗生素类药物检出。
