Practical techniques for smooth geodesic patterning of membrane structures were investigated.For the geodesic search,adjustment of the subplane of the extracted elements series was proposed,and various spline approxim...Practical techniques for smooth geodesic patterning of membrane structures were investigated.For the geodesic search,adjustment of the subplane of the extracted elements series was proposed,and various spline approximation methods were used to flatten the strip for the generation of a smooth pattern.This search approach is very simple,and the geodesic line could be easily attained by the proposed method without the need for a difficult computation method.Smooth cutting patterning can also be generated by spline approximation without the noise in discrete nodal information.Additionally,the geodesic cutting pattern saved about 21%of the required area for the catenary model due to the reduction of the curvature of the planar pattern seam line.展开更多
Culturable thermophilic microorganisms were enriched from samples collected from Lau Basin hydrothermal vents in artificial seawater medium at 45 ℃ and pH 7.0. Microbial diversities of the enriched communities were d...Culturable thermophilic microorganisms were enriched from samples collected from Lau Basin hydrothermal vents in artificial seawater medium at 45 ℃ and pH 7.0. Microbial diversities of the enriched communities were defined by performing a restriction fragment length polymorphism （RFLP） analysis of 16S rRNA gene sequences with enzymes MspI and Hin6 I. A total of 14 phylotypes have been detected by the RFLP patterns identified for 16S rRNA clone libraries of the enrichment. Analysis of sequences showed that at least four bacterial divisions presented in the clones libraries. The phyla Proteobacteria and Firmicutes were the most dominant groups. The majority of the sequences included in this analysis affiliated with Gamma Proteobacteria （71%） and Bacillus （23%）. Scanning electron micrographs revealed that there were abundant rod and coceoidal forms encased in sulphur and sodium chloride precipitate. These results revealed that there were a diversity of moderate thermophilic bacterial populations thrived in Lau Basin hydrothermal vents that were previously not detected by either molecular retrieval or strain purification techniques.展开更多
基金Project(12 High-tech Urban C22)supported by High-tech Urban Development Program,Ministry of Land,Transport and Moritime Affairs of Korea
文摘Practical techniques for smooth geodesic patterning of membrane structures were investigated.For the geodesic search,adjustment of the subplane of the extracted elements series was proposed,and various spline approximation methods were used to flatten the strip for the generation of a smooth pattern.This search approach is very simple,and the geodesic line could be easily attained by the proposed method without the need for a difficult computation method.Smooth cutting patterning can also be generated by spline approximation without the noise in discrete nodal information.Additionally,the geodesic cutting pattern saved about 21%of the required area for the catenary model due to the reduction of the curvature of the planar pattern seam line.
基金Project(200805032) supported by Grands from Scientific Research Program of Marine Public Welfare Industry of ChinaProject (DYXM-115-02-2-07) supported by China Ocean Mineral Resources R&D Association (COMRA)
文摘Culturable thermophilic microorganisms were enriched from samples collected from Lau Basin hydrothermal vents in artificial seawater medium at 45 ℃ and pH 7.0. Microbial diversities of the enriched communities were defined by performing a restriction fragment length polymorphism （RFLP） analysis of 16S rRNA gene sequences with enzymes MspI and Hin6 I. A total of 14 phylotypes have been detected by the RFLP patterns identified for 16S rRNA clone libraries of the enrichment. Analysis of sequences showed that at least four bacterial divisions presented in the clones libraries. The phyla Proteobacteria and Firmicutes were the most dominant groups. The majority of the sequences included in this analysis affiliated with Gamma Proteobacteria （71%） and Bacillus （23%）. Scanning electron micrographs revealed that there were abundant rod and coceoidal forms encased in sulphur and sodium chloride precipitate. These results revealed that there were a diversity of moderate thermophilic bacterial populations thrived in Lau Basin hydrothermal vents that were previously not detected by either molecular retrieval or strain purification techniques.
