目的研究向胫骨骨膜下单次注射转化生长因子(TGF)β1和β2后成年大白兔骨膜生发层细胞组织学的变化,探讨生长因子促进骨膜生发层细胞增殖的效果。方法 4月龄新西兰大白兔22只,分为3组,分别向两侧胫骨近端内侧骨膜下注射Tris溶液(对照组...目的研究向胫骨骨膜下单次注射转化生长因子(TGF)β1和β2后成年大白兔骨膜生发层细胞组织学的变化,探讨生长因子促进骨膜生发层细胞增殖的效果。方法 4月龄新西兰大白兔22只,分为3组,分别向两侧胫骨近端内侧骨膜下注射Tris溶液(对照组)(6只)、40 ng TGFβ1(8只)、40 ng TGFβ2(8只),2个实验组均于2个相距5 mm的部位分别注射20 ng。于术后第3、5、7及10天取材。标本均进行HE染色,对生发层厚度以及细胞密度进行观察测量及统计学分析。结果各组不同时间点生发层厚度比较,对照组注射后各时间点差异无显著性(P>0.05);注射后第5天,TGFβ1组生发层厚度(73±10)μm和TGFβ2组生发层厚度(112±13)μm较第3天明显增加;两实验组最大厚度(117±26)μm、(130±19)μm均出现在注射后第7天(P<0.05),注射后第10天与第3天差异无显著性(P>0.05)。同时间点3组间生发层厚度比较,注射后第3天、第10天差异无显著性(P>0.05);注射后第5天、第7天TGFβ1和TGFβ2组均明显高于对照组,且注射后第5天TGFβ2组厚度(112±13)μm明显大于TGFβ1组(73±10)μm(P=0.017)。细胞密度比较,3组注射后不同时间点以及3组间差异均无显著性(P>0.05)。结论单次骨膜下注射TGFβ1和TGFβ2均能一过性促进成年新西兰大白兔骨膜生发层细胞增殖。相比于TGFβ1,TGFβ2促进成年兔骨膜生发层细胞增殖效应较强。展开更多
Biological behavior is a hot issue in hepatocellular carcinoma (HCC) study. Positron emis-sion tomography (PET), a biological imaging technique, has been widely applied in many types of tumors. It is capable of noninv...Biological behavior is a hot issue in hepatocellular carcinoma (HCC) study. Positron emis-sion tomography (PET), a biological imaging technique, has been widely applied in many types of tumors. It is capable of noninvasive detection of biological behavior. Different radiotracers provide different information of HCC, including glucose/lipid metabolism, DNA synthesis, and apoptosis. In addition, radiotracer uptake relates to biological and clinical prognostic markers. In this article we review the application of several existing and novel radiotracers in PET in HCC study.展开更多
文摘目的研究向胫骨骨膜下单次注射转化生长因子(TGF)β1和β2后成年大白兔骨膜生发层细胞组织学的变化,探讨生长因子促进骨膜生发层细胞增殖的效果。方法 4月龄新西兰大白兔22只,分为3组,分别向两侧胫骨近端内侧骨膜下注射Tris溶液(对照组)(6只)、40 ng TGFβ1(8只)、40 ng TGFβ2(8只),2个实验组均于2个相距5 mm的部位分别注射20 ng。于术后第3、5、7及10天取材。标本均进行HE染色,对生发层厚度以及细胞密度进行观察测量及统计学分析。结果各组不同时间点生发层厚度比较,对照组注射后各时间点差异无显著性(P>0.05);注射后第5天,TGFβ1组生发层厚度(73±10)μm和TGFβ2组生发层厚度(112±13)μm较第3天明显增加;两实验组最大厚度(117±26)μm、(130±19)μm均出现在注射后第7天(P<0.05),注射后第10天与第3天差异无显著性(P>0.05)。同时间点3组间生发层厚度比较,注射后第3天、第10天差异无显著性(P>0.05);注射后第5天、第7天TGFβ1和TGFβ2组均明显高于对照组,且注射后第5天TGFβ2组厚度(112±13)μm明显大于TGFβ1组(73±10)μm(P=0.017)。细胞密度比较,3组注射后不同时间点以及3组间差异均无显著性(P>0.05)。结论单次骨膜下注射TGFβ1和TGFβ2均能一过性促进成年新西兰大白兔骨膜生发层细胞增殖。相比于TGFβ1,TGFβ2促进成年兔骨膜生发层细胞增殖效应较强。
基金Supported by National Natural Science Foundation of China (30770607)
文摘Biological behavior is a hot issue in hepatocellular carcinoma (HCC) study. Positron emis-sion tomography (PET), a biological imaging technique, has been widely applied in many types of tumors. It is capable of noninvasive detection of biological behavior. Different radiotracers provide different information of HCC, including glucose/lipid metabolism, DNA synthesis, and apoptosis. In addition, radiotracer uptake relates to biological and clinical prognostic markers. In this article we review the application of several existing and novel radiotracers in PET in HCC study.
