主要研究硅胶吸附L-α-GPC的动力学和热力学特性对吸附过程的控制,发现界面扩散控制速率模型1-3(1-X)2/3+2(1-X)能很好的拟合硅胶的吸附过程。通过Freundlich方程和Langmuir方程对吸附平衡模拟,得到了Langmiur方程拟合硅胶吸附L-α-GPC...主要研究硅胶吸附L-α-GPC的动力学和热力学特性对吸附过程的控制,发现界面扩散控制速率模型1-3(1-X)2/3+2(1-X)能很好的拟合硅胶的吸附过程。通过Freundlich方程和Langmuir方程对吸附平衡模拟,得到了Langmiur方程拟合硅胶吸附L-α-GPC的吸附等温线,计算出不同温度条件下的回归方程,相关系数均在0.99以上,并证实了硅胶的吸附为放热反应。同时,利用Clausius-Clapeyron方程计算出3个吸附量的硅胶吸附热力学参数分别为80、160和240 mg/g,焓变(ΔHAm)分别为24.85、23.82、22.72 k J/mol。研究结果为进一步探讨工业化硅胶柱色谱制备高纯L-α-GPC提供了基础数据和理论参考。展开更多
Diacetylenic glycerophosphatidylhydroxyethanol was synthesized from diacetylenic glycerophosphatidylcholine in the presence of phospholipase D from peanut seeds. The product structure was characterized by elemental an...Diacetylenic glycerophosphatidylhydroxyethanol was synthesized from diacetylenic glycerophosphatidylcholine in the presence of phospholipase D from peanut seeds. The product structure was characterized by elemental analysis, IR, 1H NMR and 13C NMR. The effects of the reaction conditions on the enzyme-catalyzed reaction are discussed. The results show that the enzyme （activity） was greatly increased by adding SDS. The yield of the reaction was increased compared with that reported in the literature under the conditions of pH=5.6, temperature 30 ℃, Ca2+ 40mmol/L, （n（substrate ）∶）n（SDS）=（2∶1）, reaction time 8 h. The cost was lower. The melting point of the product was 69.5 ℃.展开更多
文摘主要研究硅胶吸附L-α-GPC的动力学和热力学特性对吸附过程的控制,发现界面扩散控制速率模型1-3(1-X)2/3+2(1-X)能很好的拟合硅胶的吸附过程。通过Freundlich方程和Langmuir方程对吸附平衡模拟,得到了Langmiur方程拟合硅胶吸附L-α-GPC的吸附等温线,计算出不同温度条件下的回归方程,相关系数均在0.99以上,并证实了硅胶的吸附为放热反应。同时,利用Clausius-Clapeyron方程计算出3个吸附量的硅胶吸附热力学参数分别为80、160和240 mg/g,焓变(ΔHAm)分别为24.85、23.82、22.72 k J/mol。研究结果为进一步探讨工业化硅胶柱色谱制备高纯L-α-GPC提供了基础数据和理论参考。
文摘Diacetylenic glycerophosphatidylhydroxyethanol was synthesized from diacetylenic glycerophosphatidylcholine in the presence of phospholipase D from peanut seeds. The product structure was characterized by elemental analysis, IR, 1H NMR and 13C NMR. The effects of the reaction conditions on the enzyme-catalyzed reaction are discussed. The results show that the enzyme （activity） was greatly increased by adding SDS. The yield of the reaction was increased compared with that reported in the literature under the conditions of pH=5.6, temperature 30 ℃, Ca2+ 40mmol/L, （n（substrate ）∶）n（SDS）=（2∶1）, reaction time 8 h. The cost was lower. The melting point of the product was 69.5 ℃.
