PCR-based DNA fingerprinting, REP-PCR(repetitive element PCR), RAPD(randomly amplified polymorphic DNA) and16 S r DNA sequence analyses were used to characterize 23 Acidithiobacillus ferrooxidans strains isolated from...PCR-based DNA fingerprinting, REP-PCR(repetitive element PCR), RAPD(randomly amplified polymorphic DNA) and16 S r DNA sequence analyses were used to characterize 23 Acidithiobacillus ferrooxidans strains isolated from different environments.(GTG)5 and BOXA1 R primer were selected for REP-PCR. Twenty arbitrary primers were used for RAPD to acquire DNA profiles from A. ferrooxidans. Both RAPD and REP-PCR produce complex banding patterns and show good discriminatory ability in differentiating closely related strains of A. ferrooxidans. The strains are clustered into 4 or 5 major groups and reveal genomic diversity using(GTG)5-PCR, BOX-PCR and RAPD analysis. Phylogenetic tree based on 16 S r DNA sequences of 23 strains and related strains shows that they are clustered into two distinct groups. Twelve strains are highly related to a new Acidithiobacillus named Acidithiobacillus ferrivorans. The results indicate that PCR-based methods are effective in revealing genetic diversity among A. ferrooxidans.展开更多
Assessment of temporal and spatial variations in surface water quality is important to evaluate the health of a watershed and make necessary management decisions to control current and future pollution of receiving wa...Assessment of temporal and spatial variations in surface water quality is important to evaluate the health of a watershed and make necessary management decisions to control current and future pollution of receiving water bodies. In this work, surface water quality data for 12 physical and chemical parameters collected from 10 sampling sites in the Nenjiang River basin during the years(2012-2013) were analyzed. The results show that river water quality has significant temporal and spatial variations. Hierarchical cluster analysis(HCA) grouped 12 months into three periods(LF, MF and HF) and classified 10 monitoring sites into three regions(LP, MP and HP) based on the similarity of water quality characteristics. The principle component analysis(PCA)/factor analysis(FA) was used to recognize the factors or origins responsible for temporal and spatial water quality variations. Temporal and spatial PCA/FA revealed that the Nenjiang River water chemistry was strongly affected by rock/water interaction, hydrologic processes and anthropogenic activities. This work demonstrates that the application of HCA and PCA/FA has achieved meaningful classification based on temporal and spatial criteria.展开更多
基金Project(2010CB630901)supported by the National Basic Research Program of China
文摘PCR-based DNA fingerprinting, REP-PCR(repetitive element PCR), RAPD(randomly amplified polymorphic DNA) and16 S r DNA sequence analyses were used to characterize 23 Acidithiobacillus ferrooxidans strains isolated from different environments.(GTG)5 and BOXA1 R primer were selected for REP-PCR. Twenty arbitrary primers were used for RAPD to acquire DNA profiles from A. ferrooxidans. Both RAPD and REP-PCR produce complex banding patterns and show good discriminatory ability in differentiating closely related strains of A. ferrooxidans. The strains are clustered into 4 or 5 major groups and reveal genomic diversity using(GTG)5-PCR, BOX-PCR and RAPD analysis. Phylogenetic tree based on 16 S r DNA sequences of 23 strains and related strains shows that they are clustered into two distinct groups. Twelve strains are highly related to a new Acidithiobacillus named Acidithiobacillus ferrivorans. The results indicate that PCR-based methods are effective in revealing genetic diversity among A. ferrooxidans.
基金Project(2012ZX07501002-001)supported by Major Science and Technology Program for Water Pollution Control and Treatment of the Ministry of Science and Technology,China
文摘Assessment of temporal and spatial variations in surface water quality is important to evaluate the health of a watershed and make necessary management decisions to control current and future pollution of receiving water bodies. In this work, surface water quality data for 12 physical and chemical parameters collected from 10 sampling sites in the Nenjiang River basin during the years(2012-2013) were analyzed. The results show that river water quality has significant temporal and spatial variations. Hierarchical cluster analysis(HCA) grouped 12 months into three periods(LF, MF and HF) and classified 10 monitoring sites into three regions(LP, MP and HP) based on the similarity of water quality characteristics. The principle component analysis(PCA)/factor analysis(FA) was used to recognize the factors or origins responsible for temporal and spatial water quality variations. Temporal and spatial PCA/FA revealed that the Nenjiang River water chemistry was strongly affected by rock/water interaction, hydrologic processes and anthropogenic activities. This work demonstrates that the application of HCA and PCA/FA has achieved meaningful classification based on temporal and spatial criteria.
