目的:用高效液相法同时测定山楂叶中牡荆素-4〃-O-葡萄糖苷、牡荆素鼠李糖苷、牡荆素、芦丁、金丝桃苷的含量。方法:采用HPLC法,色谱柱:Sepax HP C18柱(4.6 mm×250mm,5μm);流动相:A为乙腈-四氢呋喃(20:1),B为0.5%甲酸溶液,采用梯...目的:用高效液相法同时测定山楂叶中牡荆素-4〃-O-葡萄糖苷、牡荆素鼠李糖苷、牡荆素、芦丁、金丝桃苷的含量。方法:采用HPLC法,色谱柱:Sepax HP C18柱(4.6 mm×250mm,5μm);流动相:A为乙腈-四氢呋喃(20:1),B为0.5%甲酸溶液,采用梯度洗脱;0～10min,A为14%～17%;10～26min,A为17%～18%;26～28min,A为18%～14%;检测波长:360nm,流速:1.0mL/min;柱温:32℃。结果:该方法加样回收率牡荆素-4″-O-葡萄糖苷:99.59%(RSD=0.50%),牡荆素鼠李糖苷:99.57%(RSD=0.96%),牡荆素:98.70%(RSD=1.10%),芦丁:99.35%(RSD=1.16%),金丝桃苷:99.28%(RSD=0.88%)。结论:该方法准确可靠,重现性好,可用于山楂叶的质量控制。展开更多
This paper reports that in the presence of cetyltrimethyl ammonium bromide as phase transfer catalyst,D-glucosamine hydrochloride reacts with 2,4-dinitrophenol to synthesize 1-O-(2’,4’-dinitrophenyl)-2-acetamido-β-...This paper reports that in the presence of cetyltrimethyl ammonium bromide as phase transfer catalyst,D-glucosamine hydrochloride reacts with 2,4-dinitrophenol to synthesize 1-O-(2’,4’-dinitrophenyl)-2-acetamido-β-D-glucoside.Chemical structure of the new compound was confirmed by elemental analysis,IR and UV.展开更多
文摘目的:用高效液相法同时测定山楂叶中牡荆素-4〃-O-葡萄糖苷、牡荆素鼠李糖苷、牡荆素、芦丁、金丝桃苷的含量。方法:采用HPLC法,色谱柱:Sepax HP C18柱(4.6 mm×250mm,5μm);流动相:A为乙腈-四氢呋喃(20:1),B为0.5%甲酸溶液,采用梯度洗脱;0～10min,A为14%～17%;10～26min,A为17%～18%;26～28min,A为18%～14%;检测波长:360nm,流速:1.0mL/min;柱温:32℃。结果:该方法加样回收率牡荆素-4″-O-葡萄糖苷:99.59%(RSD=0.50%),牡荆素鼠李糖苷:99.57%(RSD=0.96%),牡荆素:98.70%(RSD=1.10%),芦丁:99.35%(RSD=1.16%),金丝桃苷:99.28%(RSD=0.88%)。结论:该方法准确可靠,重现性好,可用于山楂叶的质量控制。
文摘This paper reports that in the presence of cetyltrimethyl ammonium bromide as phase transfer catalyst,D-glucosamine hydrochloride reacts with 2,4-dinitrophenol to synthesize 1-O-(2’,4’-dinitrophenyl)-2-acetamido-β-D-glucoside.Chemical structure of the new compound was confirmed by elemental analysis,IR and UV.
