采用对兔血红细胞的溶血实验，测定和分析了多变鱼腥藻（Anabaena variabilis OL S1）培养液在不同生长时期的溶血活性以及温度、pH和金属离子等理化因子对其溶血素活性的影响。钴果表明，任对数生长中期多变鱼腥藻开始向胞外分泌活性...采用对兔血红细胞的溶血实验，测定和分析了多变鱼腥藻（Anabaena variabilis OL S1）培养液在不同生长时期的溶血活性以及温度、pH和金属离子等理化因子对其溶血素活性的影响。钴果表明，任对数生长中期多变鱼腥藻开始向胞外分泌活性较高的溶血性毒素，在稳定期溶血活性达到最高。4℃低温几乎完全抑制了溶血素活性；在溶血反应最初的30min内，溶血活性随着温度的升高而迅速增强；但在反应持续60min后，16-37℃温度下溶血素活性趋于一致；溶血素在偏酸性条件下活性较高，而pH为7．2-9．0时活性无明显差异；Mg^2＋、Ca^2＋能显著增加其溶血活性，Cu^2＋、Mn^2＋则显著抑制其活性，Ge^2＋、Fe^3＋、Zn^2＋对其影响不大。红细胞膜组分腰固醇、鞘磷脂和卵磷脂均能竞争性结合溶血素，显著抑制溶血反成，因此可能是溶血素在红细胞膜上的作用受体。展开更多
The basic phospholipase A2 from the venom of Agkistrodon halys pallas possesses the ability to cause hemolysis in contrast to the other two phospholipase A2 from the same venom. A new form of crystals of this enzyme w...The basic phospholipase A2 from the venom of Agkistrodon halys pallas possesses the ability to cause hemolysis in contrast to the other two phospholipase A2 from the same venom. A new form of crystals of this enzyme was grown. The crystals belong to space group of P212121 with unit cell parameters of a=9. 175nm, b=10.080nm , c=2.287nm.The crystals diffract to high resolution, and are suitable for detailed structural studies. The data were collected up to 0.25nm resolutiorl using synchrotron radiation-imaging plate-Weissenberg camera system. Preliminary analysis reveals the presence of two molecules in the asymmetric uint and the molecule may pack with the smallest dimension approximately parallel to the c axis. The new crystal form is more attractive than the monoclinic one previously reported for crystallographic structure determination as it contains fewermolecules in the asymmetric unit.展开更多
文摘采用对兔血红细胞的溶血实验，测定和分析了多变鱼腥藻（Anabaena variabilis OL S1）培养液在不同生长时期的溶血活性以及温度、pH和金属离子等理化因子对其溶血素活性的影响。钴果表明，任对数生长中期多变鱼腥藻开始向胞外分泌活性较高的溶血性毒素，在稳定期溶血活性达到最高。4℃低温几乎完全抑制了溶血素活性；在溶血反应最初的30min内，溶血活性随着温度的升高而迅速增强；但在反应持续60min后，16-37℃温度下溶血素活性趋于一致；溶血素在偏酸性条件下活性较高，而pH为7．2-9．0时活性无明显差异；Mg^2＋、Ca^2＋能显著增加其溶血活性，Cu^2＋、Mn^2＋则显著抑制其活性，Ge^2＋、Fe^3＋、Zn^2＋对其影响不大。红细胞膜组分腰固醇、鞘磷脂和卵磷脂均能竞争性结合溶血素，显著抑制溶血反成，因此可能是溶血素在红细胞膜上的作用受体。
文摘The basic phospholipase A2 from the venom of Agkistrodon halys pallas possesses the ability to cause hemolysis in contrast to the other two phospholipase A2 from the same venom. A new form of crystals of this enzyme was grown. The crystals belong to space group of P212121 with unit cell parameters of a=9. 175nm, b=10.080nm , c=2.287nm.The crystals diffract to high resolution, and are suitable for detailed structural studies. The data were collected up to 0.25nm resolutiorl using synchrotron radiation-imaging plate-Weissenberg camera system. Preliminary analysis reveals the presence of two molecules in the asymmetric uint and the molecule may pack with the smallest dimension approximately parallel to the c axis. The new crystal form is more attractive than the monoclinic one previously reported for crystallographic structure determination as it contains fewermolecules in the asymmetric unit.
