AIM: To establish an LC-MS/MS method for determination of talinolol in human plasma. METHODS: Propranolol was added as internal standard before extraction. The analysis involved a Cosmosil C18 (2.0 mm×150 mm,5 μ...AIM: To establish an LC-MS/MS method for determination of talinolol in human plasma. METHODS: Propranolol was added as internal standard before extraction. The analysis involved a Cosmosil C18 (2.0 mm×150 mm,5 μm) column and the column temperature was set at 40 ℃. The mobile phase included in acetonitrile: 10 mmol ammonium formate water solution (with 0.05% formic acid) (60∶40,v/v),the flow rate was 0.2 mL/min. ESI+was performed in the MRM mode using target ions at m/z 364-308 (talinolol) and m/z 260-184 (propranolol). RESULTS: In the range of 1.25-522.40 ng/mL, the peak area of talinolol in plasma showed a good linearity,(r2=0.999). The lower limit of quantitation (LLOQ) of talinolol in plasma was 1.25 ng/mL. The extracted recovery was >80%, The relative recovery was 95%-110%,the intra-and inter-day RSD were <15%. CONCLUSION: It can be used for determination of talinolol tablets plasma concentration and pharmacokinetic study as a sensitive and simple method.展开更多
文摘AIM: To establish an LC-MS/MS method for determination of talinolol in human plasma. METHODS: Propranolol was added as internal standard before extraction. The analysis involved a Cosmosil C18 (2.0 mm×150 mm,5 μm) column and the column temperature was set at 40 ℃. The mobile phase included in acetonitrile: 10 mmol ammonium formate water solution (with 0.05% formic acid) (60∶40,v/v),the flow rate was 0.2 mL/min. ESI+was performed in the MRM mode using target ions at m/z 364-308 (talinolol) and m/z 260-184 (propranolol). RESULTS: In the range of 1.25-522.40 ng/mL, the peak area of talinolol in plasma showed a good linearity,(r2=0.999). The lower limit of quantitation (LLOQ) of talinolol in plasma was 1.25 ng/mL. The extracted recovery was >80%, The relative recovery was 95%-110%,the intra-and inter-day RSD were <15%. CONCLUSION: It can be used for determination of talinolol tablets plasma concentration and pharmacokinetic study as a sensitive and simple method.
