【目的】探索槲皮素对母羊卵泡发育、免疫及抗氧化功能、卵泡液生殖激素分泌和卵巢类固醇生成相关基因的影响。【方法】本试验选取体重相近、经产蒙古羊母羊30只,随机分为2组:对照组和槲皮素组,对照组饲喂基础饲粮,槲皮素组每只羊在基...【目的】探索槲皮素对母羊卵泡发育、免疫及抗氧化功能、卵泡液生殖激素分泌和卵巢类固醇生成相关基因的影响。【方法】本试验选取体重相近、经产蒙古羊母羊30只,随机分为2组:对照组和槲皮素组,对照组饲喂基础饲粮,槲皮素组每只羊在基础饲粮中添加槲皮素7 g/d,每组3个重复,每个重复5只羊单独一栏饲养。试验采用同期发情处理,在第2个情期第13天采集血液用于抗氧化指标和生殖激素指标检测,采集母羊卵巢组织用于类固醇激素生成相关基因表达的检测。【结果】①相比于对照组,槲皮素组母羊的大卵泡和中卵泡数显著增加(P<0.05),血清中白细胞介素-6、肿瘤坏死因子、免疫球蛋白G和免疫球蛋白A的水平极显著或显著降低(P<0.01;P<0.05),同时血清中总抗氧化能力、超氧化物歧化酶和谷胱甘肽过氧化物酶的活力极显著或显著升高(P<0.01;P<0.05)。②相比于对照组,槲皮素组母羊大卵泡内雌二醇、孕酮、促卵泡生成素和促性腺激素释放激素水平极显著或显著升高(P<0.01;P<0.05),中等卵泡内雌二醇和促卵泡生成素水平极显著升高(P<0.01);各级卵泡内促黄体生成素浓度均显著或极显著降低(P<0.05;P<0.01)。③相比于对照组,饲粮添加槲皮素极显著上调了母羊卵巢类固醇激素合成急性调节蛋白(steroidogenic acute regulatory protein,STAR)和细胞色素P450家族19A成员1(cytochrome P450 family 19 subfamily A member 1,CYP19A1)的mRNA表达(P<0.01)。【结论】饲粮添加7 g/d槲皮素可有效提高母羊抗炎症和抗氧化功能,并通过调控卵泡液生殖激素分泌和卵巢类固醇生成相关基因表达,进而影响母羊卵泡发育。展开更多
An attempt was made to build up a thick and compact oxide layer rapidly by pre-treating the Pb-Ag-Nd anode in fluoride-containing H2SO4 solution. The passivation reaction of Pb-Ag-Nd anode during pre-treatment process...An attempt was made to build up a thick and compact oxide layer rapidly by pre-treating the Pb-Ag-Nd anode in fluoride-containing H2SO4 solution. The passivation reaction of Pb-Ag-Nd anode during pre-treatment process was investigated using cyclic voltammetry, linear scanning voltammetry, environmental scanning electron microscopy and X-ray diffraction analysis. The results show that Pb F2 and PbSO4 are formed near the potential of Pb/PbSO4 couple. The pre-treatment in fluoride-containing H2SO4 solution contributes to the formation of a thick, compact and adherent passive film. Furthermore, pre-treatment in fluoride-containing H2SO4 solution also facilitates the formation of PbO2 on the anodic layer, and the reason could be attributed to the formation of more PbF2 and PbSO4 during the pre-treatment which tend to transform to PbO2 during the following electrowinning process. In addition, the anodic layer on anode with pre-treatment in fluoride-containing H2SO4 solution is thick and compact, and its predominant composition is β-PbO2. In summary, the pre-treatment in fluoride-containing H2SO4 solution benefits the formation of a desirable protective layer in a short time.展开更多
文摘【目的】探索槲皮素对母羊卵泡发育、免疫及抗氧化功能、卵泡液生殖激素分泌和卵巢类固醇生成相关基因的影响。【方法】本试验选取体重相近、经产蒙古羊母羊30只,随机分为2组:对照组和槲皮素组,对照组饲喂基础饲粮,槲皮素组每只羊在基础饲粮中添加槲皮素7 g/d,每组3个重复,每个重复5只羊单独一栏饲养。试验采用同期发情处理,在第2个情期第13天采集血液用于抗氧化指标和生殖激素指标检测,采集母羊卵巢组织用于类固醇激素生成相关基因表达的检测。【结果】①相比于对照组,槲皮素组母羊的大卵泡和中卵泡数显著增加(P<0.05),血清中白细胞介素-6、肿瘤坏死因子、免疫球蛋白G和免疫球蛋白A的水平极显著或显著降低(P<0.01;P<0.05),同时血清中总抗氧化能力、超氧化物歧化酶和谷胱甘肽过氧化物酶的活力极显著或显著升高(P<0.01;P<0.05)。②相比于对照组,槲皮素组母羊大卵泡内雌二醇、孕酮、促卵泡生成素和促性腺激素释放激素水平极显著或显著升高(P<0.01;P<0.05),中等卵泡内雌二醇和促卵泡生成素水平极显著升高(P<0.01);各级卵泡内促黄体生成素浓度均显著或极显著降低(P<0.05;P<0.01)。③相比于对照组,饲粮添加槲皮素极显著上调了母羊卵巢类固醇激素合成急性调节蛋白(steroidogenic acute regulatory protein,STAR)和细胞色素P450家族19A成员1(cytochrome P450 family 19 subfamily A member 1,CYP19A1)的mRNA表达(P<0.01)。【结论】饲粮添加7 g/d槲皮素可有效提高母羊抗炎症和抗氧化功能,并通过调控卵泡液生殖激素分泌和卵巢类固醇生成相关基因表达,进而影响母羊卵泡发育。
基金Projects(51204208,51374240)supported by the National Natural Science Foundation of ChinaProject(2014zzts028)supported by the Fundamental Research Funds for the Central Universities of Central South University,China
文摘An attempt was made to build up a thick and compact oxide layer rapidly by pre-treating the Pb-Ag-Nd anode in fluoride-containing H2SO4 solution. The passivation reaction of Pb-Ag-Nd anode during pre-treatment process was investigated using cyclic voltammetry, linear scanning voltammetry, environmental scanning electron microscopy and X-ray diffraction analysis. The results show that Pb F2 and PbSO4 are formed near the potential of Pb/PbSO4 couple. The pre-treatment in fluoride-containing H2SO4 solution contributes to the formation of a thick, compact and adherent passive film. Furthermore, pre-treatment in fluoride-containing H2SO4 solution also facilitates the formation of PbO2 on the anodic layer, and the reason could be attributed to the formation of more PbF2 and PbSO4 during the pre-treatment which tend to transform to PbO2 during the following electrowinning process. In addition, the anodic layer on anode with pre-treatment in fluoride-containing H2SO4 solution is thick and compact, and its predominant composition is β-PbO2. In summary, the pre-treatment in fluoride-containing H2SO4 solution benefits the formation of a desirable protective layer in a short time.
