The method for the separation of clorprenaline hydrochloride enantiomers was developed using reversed phase high-performance liquid chromatography(RP-HPLC)with carboxymethyl-β-cyclodextrin(CM-β-CD)as chiral mobile p...The method for the separation of clorprenaline hydrochloride enantiomers was developed using reversed phase high-performance liquid chromatography(RP-HPLC)with carboxymethyl-β-cyclodextrin(CM-β-CD)as chiral mobile phase additive.The effects of concentration of CM-β-CD,organic solvents(type and concentration)and flow rate on the enantiomers separation was studied systematically.Separation was performed on a Diamond C18 column.The best separation was obtained with methanol-acetonitrile-water(80∶10∶10,v/v/v)mixed with 0.40g/L CM-β-CD as mobile.The optimal flow rate was 0.20 mL/min.The detection wavelength was set at 216 nm.Under the optimized conditions,the resolution of enantiomers was 1.60 in 30 minutes,The method was simple and fast with small relative standard deviations.展开更多
文摘The method for the separation of clorprenaline hydrochloride enantiomers was developed using reversed phase high-performance liquid chromatography(RP-HPLC)with carboxymethyl-β-cyclodextrin(CM-β-CD)as chiral mobile phase additive.The effects of concentration of CM-β-CD,organic solvents(type and concentration)and flow rate on the enantiomers separation was studied systematically.Separation was performed on a Diamond C18 column.The best separation was obtained with methanol-acetonitrile-water(80∶10∶10,v/v/v)mixed with 0.40g/L CM-β-CD as mobile.The optimal flow rate was 0.20 mL/min.The detection wavelength was set at 216 nm.Under the optimized conditions,the resolution of enantiomers was 1.60 in 30 minutes,The method was simple and fast with small relative standard deviations.
