The new emerging stems and buds of poplar 110(Populus deltoids×P.maximowiczii cv ’Eridano’) were used as explants and incubated on MS media supplemented with various hormone combinations.Once the successive and...The new emerging stems and buds of poplar 110(Populus deltoids×P.maximowiczii cv ’Eridano’) were used as explants and incubated on MS media supplemented with various hormone combinations.Once the successive and rooting plants were obtained through selection of cultural medium formula,leaves and internodals were excised from tubed-culture planlets and induced to form regenerated plants.The result showed MS+6-BA 0.5 mg·L-1+NAA 0.1 mg·L-1+TDZ 0.02 mg·L-1 was beneficial for leaves to inducing adventitious buds;The proper medium for using internodals as explants was MS+6-BA 0.3 mg·L-1+NAA 0.05 mg·L-1+TDZ 0.02 mg·L-1;while the medium of MS+6-BA 0.3 mg·L-1+NAA 0.1 mg·L-1 was proper to culturing the stronger tubed-planlets.The optimum medium for producing roots was 1/2MS+IBA 0.5 mg·L-1.The effect of different incising mode and growth state of leaves and internodals on inducing adventitious was discussed.In vitro vegetative propagation and plant rooting were optimized to establish the high-efficient tissue culture reproduction system of poplar 110,which make it possible for tree breeding and genetic transformation of this species.展开更多
文摘The new emerging stems and buds of poplar 110(Populus deltoids×P.maximowiczii cv ’Eridano’) were used as explants and incubated on MS media supplemented with various hormone combinations.Once the successive and rooting plants were obtained through selection of cultural medium formula,leaves and internodals were excised from tubed-culture planlets and induced to form regenerated plants.The result showed MS+6-BA 0.5 mg·L-1+NAA 0.1 mg·L-1+TDZ 0.02 mg·L-1 was beneficial for leaves to inducing adventitious buds;The proper medium for using internodals as explants was MS+6-BA 0.3 mg·L-1+NAA 0.05 mg·L-1+TDZ 0.02 mg·L-1;while the medium of MS+6-BA 0.3 mg·L-1+NAA 0.1 mg·L-1 was proper to culturing the stronger tubed-planlets.The optimum medium for producing roots was 1/2MS+IBA 0.5 mg·L-1.The effect of different incising mode and growth state of leaves and internodals on inducing adventitious was discussed.In vitro vegetative propagation and plant rooting were optimized to establish the high-efficient tissue culture reproduction system of poplar 110,which make it possible for tree breeding and genetic transformation of this species.
