The samples of minks from several main mink farming areas were detected by the methods of counter immunoelectrophoresis(CIEP)and polymerase chain reaction (PCR)and some data about Aleutian mink disease parvovirus(ADV)...The samples of minks from several main mink farming areas were detected by the methods of counter immunoelectrophoresis(CIEP)and polymerase chain reaction (PCR)and some data about Aleutian mink disease parvovirus(ADV)infection in farmed mink was obtained. Partial VP2 genes,which include a hypervariable region, of 16 ADV strains isolated were separately amplified by PCR and sequenced. Comparison and analysis of these genes sequences and the related sequences of ADV-G,ADV-Utah1 and ADV-K that reported in the USA and European had been completed. The result shows that : firstly, the closed relationship exists between domestic ADV isolates and foreign ones,and the domestic AD maybe introduced from foreign countries. Secondly, the ADV isolates we obtained have a high genetic diversity. DL1, DL2, MS3 and MS5 may be some new types of ADV because of so obvious differences compared with foreign isolates in the genes sequences.展开更多
文摘The samples of minks from several main mink farming areas were detected by the methods of counter immunoelectrophoresis(CIEP)and polymerase chain reaction (PCR)and some data about Aleutian mink disease parvovirus(ADV)infection in farmed mink was obtained. Partial VP2 genes,which include a hypervariable region, of 16 ADV strains isolated were separately amplified by PCR and sequenced. Comparison and analysis of these genes sequences and the related sequences of ADV-G,ADV-Utah1 and ADV-K that reported in the USA and European had been completed. The result shows that : firstly, the closed relationship exists between domestic ADV isolates and foreign ones,and the domestic AD maybe introduced from foreign countries. Secondly, the ADV isolates we obtained have a high genetic diversity. DL1, DL2, MS3 and MS5 may be some new types of ADV because of so obvious differences compared with foreign isolates in the genes sequences.
