p-t-Butyl-calixarene bonded silica stationary phase(CBS) for high performance liquid chromatography was prepared via condensation reaction on the surface of solid .The stationary phase was characterized by means of el...p-t-Butyl-calixarene bonded silica stationary phase(CBS) for high performance liquid chromatography was prepared via condensation reaction on the surface of solid .The stationary phase was characterized by means of elemental analysis and FTIR. The chromatographic performance of CBS was studied by using disubstituted benzenes as the solutes and methanol-water or methanol-buffer as the mobile phases, and compared with that obtained on CPS and ODS. The results show that the chromrtographic process can proceed in various retention mechanism, such as π-π, hydrogen-bonding, charge-transfer and inclusion complexation, besides hydrophobic interaction.展开更多
Two chiral stationary phases(BCDs and YBCDs) were prepared by bonding β-cyclodextrin derivative with α-Schiff base group from β-cyclodextrin monoaldehyde to the sillica gel via 3-glyci-{dyloxypropyltrimethoxysilane...Two chiral stationary phases(BCDs and YBCDs) were prepared by bonding β-cyclodextrin derivative with α-Schiff base group from β-cyclodextrin monoaldehyde to the sillica gel via 3-glyci-{dyloxypropyltrimethoxysilane} as a coupling agent. Using acetonitrile-TEAA as mobile phase, good enantiomeric resolutions were obtained for DL-aminoacide on BCDs and YBCDs, including Leucine, Threonine and Valine. It is also observed that the higher separation factor of threonine was at pH {7.11} for both of the column, and the optimal column temperatures of Leucine appeared at 30 ℃ or {40 ℃}, respectively for YBCDs and BCDs.展开更多
文摘p-t-Butyl-calixarene bonded silica stationary phase(CBS) for high performance liquid chromatography was prepared via condensation reaction on the surface of solid .The stationary phase was characterized by means of elemental analysis and FTIR. The chromatographic performance of CBS was studied by using disubstituted benzenes as the solutes and methanol-water or methanol-buffer as the mobile phases, and compared with that obtained on CPS and ODS. The results show that the chromrtographic process can proceed in various retention mechanism, such as π-π, hydrogen-bonding, charge-transfer and inclusion complexation, besides hydrophobic interaction.
文摘Two chiral stationary phases(BCDs and YBCDs) were prepared by bonding β-cyclodextrin derivative with α-Schiff base group from β-cyclodextrin monoaldehyde to the sillica gel via 3-glyci-{dyloxypropyltrimethoxysilane} as a coupling agent. Using acetonitrile-TEAA as mobile phase, good enantiomeric resolutions were obtained for DL-aminoacide on BCDs and YBCDs, including Leucine, Threonine and Valine. It is also observed that the higher separation factor of threonine was at pH {7.11} for both of the column, and the optimal column temperatures of Leucine appeared at 30 ℃ or {40 ℃}, respectively for YBCDs and BCDs.
