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葡萄糖氧化酶氧耗速率法测定葡萄糖最佳条件探讨 被引量:1
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作者 刘福成 李学仁 沈瑛 《中国医学科学院学报》 CAS CSCD 北大核心 1997年第1期75-77,共3页
We studied optimal conditions for the determination of oxygen consumption rate (OCR).The assay, with a working range up to 35 mmol/L, had an intra-assay coefficient of variation of 0. 7%~3. 52%, and the inter-assay c... We studied optimal conditions for the determination of oxygen consumption rate (OCR).The assay, with a working range up to 35 mmol/L, had an intra-assay coefficient of variation of 0. 7%~3. 52%, and the inter-assay coefficient of variation was 4. 86%. In spiking experiments, the recovery range was 98. 9%~101 %. The assay was compared with CX3 (Beckman Co.). A linear regression analysis yielded a slope of 1. 05, an inercept of 3. 82 and a correlation coefficient of 0. 999. The serum of turbidity above + + had a negative interference on the assay (>3. 4%). The obvious interference of hemolysis, icterus and vitamin C to the assay were not observed. 展开更多
关键词 葡萄糖 化酶 氧耗速率法 测定 条件
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