以宁夏贺兰山东麓5个不同子产区石嘴山市、银川市贺兰县、西夏区、永宁县和吴忠市红寺堡区的17款赤霞珠干红葡萄酒为研究对象,采用顶空固相微萃取与气相色谱-质谱联用法(headpace solid-phase microextraction and gas chromatography-m...以宁夏贺兰山东麓5个不同子产区石嘴山市、银川市贺兰县、西夏区、永宁县和吴忠市红寺堡区的17款赤霞珠干红葡萄酒为研究对象,采用顶空固相微萃取与气相色谱-质谱联用法(headpace solid-phase microextraction and gas chromatography-mass spectrometry,HS-SPME-GC-MS),分析酒样的香气物质以及气味活性值(odor activity value,OAV),确定具有感官贡献的香气物质,结合主成分分析研究5个子产区之间的相关性。结果表明,酒样香气物质在组成上具有一致性,主要是醇类和酯类物质。此外,大马士酮香气贡献最为突出,使得宁夏贺兰山东麓赤霞珠干红葡萄酒香气主要具有花香和果香特征。主成分分析结果表明,利用植物香指数和脂香指数可区分不同子产区的香气特征,辛酸甲酯、乙酸苯乙酯、月桂酸乙酯;2,4-二叔丁基苯酚;苯乙醇;大马士酮和癸酸乙酯;乙酸异戊酯分别对石嘴山市、银川市贺兰县、西夏区、永宁县和吴忠市红寺堡区葡萄酒香气有重要贡献。聚类结果表明,石嘴山市和银川市西夏区葡萄酒香气更为接近。展开更多
This study is intended to explore the chemical differences of Acori Tatarinowii Rhizoma (ATR) samples collected from two habitats, Sichuan and Anhui provinces, China. Gas chromatography-mass spectrometry (GC-MS) w...This study is intended to explore the chemical differences of Acori Tatarinowii Rhizoma (ATR) samples collected from two habitats, Sichuan and Anhui provinces, China. Gas chromatography-mass spectrometry (GC-MS) was applied to establishing the quantitative chemical fingerprints of ATRs. A total of 104 volatile compounds were identified and quantified with the information of mass spectra and retention index (RI). Furthermore, least absolute shrinkage and selection operator (LASSO), a sparse regularization method, combined with subsampling was employed to improve the classification ability of partial least squares-discriminant analysis (PLS-DA). After variable selection by LASSO, three chemical markers,β-elemene, α-selinene and α-asarone, were identified for the discrimination of ATRs from two habitats, and the total classification correct rate was increased from 82.76% to 96.55%. The proposed LASSO-PLS-DA method can serve as an efficient strategy for screening marked chemical components and geo-herbalism research of traditional Chinese medicines.展开更多
文摘以宁夏贺兰山东麓5个不同子产区石嘴山市、银川市贺兰县、西夏区、永宁县和吴忠市红寺堡区的17款赤霞珠干红葡萄酒为研究对象,采用顶空固相微萃取与气相色谱-质谱联用法(headpace solid-phase microextraction and gas chromatography-mass spectrometry,HS-SPME-GC-MS),分析酒样的香气物质以及气味活性值(odor activity value,OAV),确定具有感官贡献的香气物质,结合主成分分析研究5个子产区之间的相关性。结果表明,酒样香气物质在组成上具有一致性,主要是醇类和酯类物质。此外,大马士酮香气贡献最为突出,使得宁夏贺兰山东麓赤霞珠干红葡萄酒香气主要具有花香和果香特征。主成分分析结果表明,利用植物香指数和脂香指数可区分不同子产区的香气特征,辛酸甲酯、乙酸苯乙酯、月桂酸乙酯;2,4-二叔丁基苯酚;苯乙醇;大马士酮和癸酸乙酯;乙酸异戊酯分别对石嘴山市、银川市贺兰县、西夏区、永宁县和吴忠市红寺堡区葡萄酒香气有重要贡献。聚类结果表明,石嘴山市和银川市西夏区葡萄酒香气更为接近。
基金Project(21465016)supported by the National Natural Foundation of China
文摘This study is intended to explore the chemical differences of Acori Tatarinowii Rhizoma (ATR) samples collected from two habitats, Sichuan and Anhui provinces, China. Gas chromatography-mass spectrometry (GC-MS) was applied to establishing the quantitative chemical fingerprints of ATRs. A total of 104 volatile compounds were identified and quantified with the information of mass spectra and retention index (RI). Furthermore, least absolute shrinkage and selection operator (LASSO), a sparse regularization method, combined with subsampling was employed to improve the classification ability of partial least squares-discriminant analysis (PLS-DA). After variable selection by LASSO, three chemical markers,β-elemene, α-selinene and α-asarone, were identified for the discrimination of ATRs from two habitats, and the total classification correct rate was increased from 82.76% to 96.55%. The proposed LASSO-PLS-DA method can serve as an efficient strategy for screening marked chemical components and geo-herbalism research of traditional Chinese medicines.