A two-dimensional separation platform of capillary isoelectric focusing(CIEF)-capillary zone electrophoresis(CZE) for proteins was constructed by a microdialysis hollow fiber interface. The interface provided ease of ...A two-dimensional separation platform of capillary isoelectric focusing(CIEF)-capillary zone electrophoresis(CZE) for proteins was constructed by a microdialysis hollow fiber interface. The interface provided ease of fabrication,speed of mass delivering and convenience of column switching. Chrome c was selected to demonstrate the 2D separation systems feasibility and resolving performance. The 2D separation system was found to greatly increase the resolving power and overall peak capacity over those obtained for either dimension alone.展开更多
An important interface modification for capillary isoelectric focusing mass spectrometry(CIEF MS) is reported. A capillary needle with a sheath flow tube pre mounted in one device serves as both the CIEF cathodic end ...An important interface modification for capillary isoelectric focusing mass spectrometry(CIEF MS) is reported. A capillary needle with a sheath flow tube pre mounted in one device serves as both the CIEF cathodic end and the ESI interface. The needle was inserted into the cathode reservoir at the focusing stage, and then removed to the front of sampler orifice of MS at the detection stage. Therefore, the shut off of the high voltage is not necessary and a better focusing protein zone is maintained during the ESI stage. The optimization of the experimental condition has been investigated and the two dimensional image of the E. coli proteins has been obtained, which is comparable with that from a 2D gel.展开更多
A simple method for mobilizing the focused zones of capillary isoelectric focusing(CIEF) for single-point detectors was developed, in which a linear polyacrylamide(LPA) coated capillary was connected with an uncoated ...A simple method for mobilizing the focused zones of capillary isoelectric focusing(CIEF) for single-point detectors was developed, in which a linear polyacrylamide(LPA) coated capillary was connected with an uncoated capillary by an etched porous joint. The etched section allows electrical conductivity but blocks the hydrodynamic flow. The focused zones of mixed proteins was mobilized by this method successfully. Compared with the traditional gravity mobilization step, it was found that this method can mobilize the focused zones more effectively without sacrificing separation efficiency.展开更多
文摘A two-dimensional separation platform of capillary isoelectric focusing(CIEF)-capillary zone electrophoresis(CZE) for proteins was constructed by a microdialysis hollow fiber interface. The interface provided ease of fabrication,speed of mass delivering and convenience of column switching. Chrome c was selected to demonstrate the 2D separation systems feasibility and resolving performance. The 2D separation system was found to greatly increase the resolving power and overall peak capacity over those obtained for either dimension alone.
文摘An important interface modification for capillary isoelectric focusing mass spectrometry(CIEF MS) is reported. A capillary needle with a sheath flow tube pre mounted in one device serves as both the CIEF cathodic end and the ESI interface. The needle was inserted into the cathode reservoir at the focusing stage, and then removed to the front of sampler orifice of MS at the detection stage. Therefore, the shut off of the high voltage is not necessary and a better focusing protein zone is maintained during the ESI stage. The optimization of the experimental condition has been investigated and the two dimensional image of the E. coli proteins has been obtained, which is comparable with that from a 2D gel.
文摘A simple method for mobilizing the focused zones of capillary isoelectric focusing(CIEF) for single-point detectors was developed, in which a linear polyacrylamide(LPA) coated capillary was connected with an uncoated capillary by an etched porous joint. The etched section allows electrical conductivity but blocks the hydrodynamic flow. The focused zones of mixed proteins was mobilized by this method successfully. Compared with the traditional gravity mobilization step, it was found that this method can mobilize the focused zones more effectively without sacrificing separation efficiency.
基金This research was conductedas part of the Special Research Program‘Forest Ecosystem Restoration(SF008)’funded by the Austrian Science Foundationandthe Ministry of Agriculture and Forestry
