Microwave irradiation was firstly attempted to prepare molecularly imprinted polymers(MIPs)monoliths,which were in situ prepared rapidly with methacrylic acid(MAA)as a functional monomer,ethylene glycol dimethacrylate...Microwave irradiation was firstly attempted to prepare molecularly imprinted polymers(MIPs)monoliths,which were in situ prepared rapidly with methacrylic acid(MAA)as a functional monomer,ethylene glycol dimethacrylate(EDMA)as a cross-linking agent,toluene and isooctane as the porogenic solvents and AIBN as an initiator.The baseline separation of isomers of hydroxybenzoic acid was achieved on these monolithic columns with 4-HBA as the template,but not on the blank polymer.Furthermore,some neutral compounds could also be baseline-separated on the imprinted polymer columns in the modes of pressure-driven capillary electrochromatography and low-pressure driven capillary electrochromatography.It was shown that the monoliths were not only used as the molecularly imprinted polymers but also as the reverse phase chromatographic material.展开更多
A novel monolithic stationary phase with long alkyl chain ligands was introduced and evaluated in pressurized capillary electrochromatography(p-CEC) by using a mixture of neutral and charged compounds. The capillary m...A novel monolithic stationary phase with long alkyl chain ligands was introduced and evaluated in pressurized capillary electrochromatography(p-CEC) by using a mixture of neutral and charged compounds. The capillary monolithic-column was prepared by in situ copolymerization of ethylene dimethacrylate,alkyl alcohol and 2-acrylamido-2-methyl-1-propanesulfonic acid in a quadruple porogenic solvent consisting of 1,4-butanediol/cyclohexanol/docecanol/water. Effects of experimental parameters in p-CEC,such as pressure and electrical field strength,on the separation were investigated. The baseline separation of the components in the mixture was achieved. The monolithic column can be used as an alternative to the conventional capillary column such as the reversed phase ODS.展开更多
以1.5μm无孔硅胶颗粒(non-po rou s s ilica,NPS)为固定相,采用电压和压力联合驱动流动相,用反相梯度加压毛细管电色谱(p-CEC)在7.5m in内实现了核糖核酸酶A、细胞色素C、溶菌酶和肌红蛋白等4种蛋白质的快速、高效的分离。比较了梯度...以1.5μm无孔硅胶颗粒(non-po rou s s ilica,NPS)为固定相,采用电压和压力联合驱动流动相,用反相梯度加压毛细管电色谱(p-CEC)在7.5m in内实现了核糖核酸酶A、细胞色素C、溶菌酶和肌红蛋白等4种蛋白质的快速、高效的分离。比较了梯度加压毛细管电色谱和微柱液相色谱(μ-HPLC)分离蛋白质的结果,同时考察了固定相、离子对试剂三氟醋酸(TFA)浓度和电压等条件对梯度加压毛细管电色谱分离蛋白质的影响。结果表明,梯度p-CEC可以通过调节电压精细调节带电溶质的保留,提高分离选择性,缩短分离时间,得到较高的柱效。该方法在蛋白质分离分析及蛋白质组学的研究中具有很大的应用潜力,为高效快速地分离蛋白质开辟了新的途径。展开更多
文摘Microwave irradiation was firstly attempted to prepare molecularly imprinted polymers(MIPs)monoliths,which were in situ prepared rapidly with methacrylic acid(MAA)as a functional monomer,ethylene glycol dimethacrylate(EDMA)as a cross-linking agent,toluene and isooctane as the porogenic solvents and AIBN as an initiator.The baseline separation of isomers of hydroxybenzoic acid was achieved on these monolithic columns with 4-HBA as the template,but not on the blank polymer.Furthermore,some neutral compounds could also be baseline-separated on the imprinted polymer columns in the modes of pressure-driven capillary electrochromatography and low-pressure driven capillary electrochromatography.It was shown that the monoliths were not only used as the molecularly imprinted polymers but also as the reverse phase chromatographic material.
文摘A novel monolithic stationary phase with long alkyl chain ligands was introduced and evaluated in pressurized capillary electrochromatography(p-CEC) by using a mixture of neutral and charged compounds. The capillary monolithic-column was prepared by in situ copolymerization of ethylene dimethacrylate,alkyl alcohol and 2-acrylamido-2-methyl-1-propanesulfonic acid in a quadruple porogenic solvent consisting of 1,4-butanediol/cyclohexanol/docecanol/water. Effects of experimental parameters in p-CEC,such as pressure and electrical field strength,on the separation were investigated. The baseline separation of the components in the mixture was achieved. The monolithic column can be used as an alternative to the conventional capillary column such as the reversed phase ODS.
文摘以1.5μm无孔硅胶颗粒(non-po rou s s ilica,NPS)为固定相,采用电压和压力联合驱动流动相,用反相梯度加压毛细管电色谱(p-CEC)在7.5m in内实现了核糖核酸酶A、细胞色素C、溶菌酶和肌红蛋白等4种蛋白质的快速、高效的分离。比较了梯度加压毛细管电色谱和微柱液相色谱(μ-HPLC)分离蛋白质的结果,同时考察了固定相、离子对试剂三氟醋酸(TFA)浓度和电压等条件对梯度加压毛细管电色谱分离蛋白质的影响。结果表明,梯度p-CEC可以通过调节电压精细调节带电溶质的保留,提高分离选择性,缩短分离时间,得到较高的柱效。该方法在蛋白质分离分析及蛋白质组学的研究中具有很大的应用潜力,为高效快速地分离蛋白质开辟了新的途径。
