为监测畜产品中克伦特罗和沙丁胺醇残留,建立了一种适于猪肝等生物组织样品的毛细管电泳-质谱联用分析方法。优化了克伦特罗和沙丁胺醇两种β-激动素的毛细管电泳分离和质谱检测的条件。样品经预处理后,用毛细管电泳-质谱联用分离分析,...为监测畜产品中克伦特罗和沙丁胺醇残留,建立了一种适于猪肝等生物组织样品的毛细管电泳-质谱联用分析方法。优化了克伦特罗和沙丁胺醇两种β-激动素的毛细管电泳分离和质谱检测的条件。样品经预处理后,用毛细管电泳-质谱联用分离分析,电泳分离时间小于6 m in;在最佳实验条件下,克伦特罗和沙丁胺醇的检出限分别为0.4和0.3μg/kg。方法灵敏度高、特异性强,测定结果准确可靠,可用于畜产品中克伦特罗和沙丁胺醇残留的确证性检测。展开更多
With the sequencing of human genome almost complete, human genome project enters the post-genome-sequencing era. Compared to genomics, the analysis of proteome is rather difficult since in human cells there are around...With the sequencing of human genome almost complete, human genome project enters the post-genome-sequencing era. Compared to genomics, the analysis of proteome is rather difficult since in human cells there are around 200000 proteins, which are expressed at any time at different levels of concentration with a wide dynamic range. In addition, a given proteome is not static but dynamic, being defined by a combination of the cell's genome, environment, and even its history. To meet the higher and higher requirements from proteome analysis, in our recent work, we have developed some capillary electrophoresis (CE)-based techniques for the analysis of complex protein samples.展开更多
文摘为监测畜产品中克伦特罗和沙丁胺醇残留,建立了一种适于猪肝等生物组织样品的毛细管电泳-质谱联用分析方法。优化了克伦特罗和沙丁胺醇两种β-激动素的毛细管电泳分离和质谱检测的条件。样品经预处理后,用毛细管电泳-质谱联用分离分析,电泳分离时间小于6 m in;在最佳实验条件下,克伦特罗和沙丁胺醇的检出限分别为0.4和0.3μg/kg。方法灵敏度高、特异性强,测定结果准确可靠,可用于畜产品中克伦特罗和沙丁胺醇残留的确证性检测。
文摘With the sequencing of human genome almost complete, human genome project enters the post-genome-sequencing era. Compared to genomics, the analysis of proteome is rather difficult since in human cells there are around 200000 proteins, which are expressed at any time at different levels of concentration with a wide dynamic range. In addition, a given proteome is not static but dynamic, being defined by a combination of the cell's genome, environment, and even its history. To meet the higher and higher requirements from proteome analysis, in our recent work, we have developed some capillary electrophoresis (CE)-based techniques for the analysis of complex protein samples.