Using one step solid-phase extraction for urine pretreatment and a new sample focusing mode-dynamic pH junction injection for increasing sensitivity, a simple and sensitive method for the analysis of urinary 8OHdG by ...Using one step solid-phase extraction for urine pretreatment and a new sample focusing mode-dynamic pH junction injection for increasing sensitivity, a simple and sensitive method for the analysis of urinary 8OHdG by capillary electrophoresis with end-column amprometric detection has been developed. The limit of detection was 20 nmol/L(signal to noise ratio S/N=3). The urinary concentration of 8OHdG in nine healthy persons and 28 cancer patients was determined. It was found that urinary concentration of 8OHdG in cancer patients was significantly higher than that in healthy persons[(35.26±27.96) nmol/L) vs. (13.51±5.08) nmol/L, P<0.05], the result demonstrated that 8OHdG may be a good biomarker for measurement of oxidative DNA damage in cancer patients. Furthermore, the excretion levels of urinary 8OHdG from cancer patients receiving surgical therapy are investigated, and the results demonstrated that the smoking has a strong effect on 8OHdG content.展开更多
首次采用毛细管电泳-安培检测法(CE-AD)同时测定了苯丙酮尿症(Phenylketonuria,PKU)患者尿液中的两种标志物:具有电活性的苯丙酮酸及非电活性的苯乙酸。考察了运行液添加剂、SDS浓度、工作电极电位等因素对分离和检测的影响。在优化实...首次采用毛细管电泳-安培检测法(CE-AD)同时测定了苯丙酮尿症(Phenylketonuria,PKU)患者尿液中的两种标志物:具有电活性的苯丙酮酸及非电活性的苯乙酸。考察了运行液添加剂、SDS浓度、工作电极电位等因素对分离和检测的影响。在优化实验条件下,两种标志物在15 m in内可实现基线分离。苯丙酮酸和苯乙酸的检出限(S/N=3)分别为0.50、0.92 mg/L。苯丙酮酸、苯乙酸浓度与峰高分别在3.0～1.0×103mg/L、2.0～5.0×103mg/L范围内呈良好线性,峰高与迁移时间的相对标准偏差分别为4.4%、3.2%(苯丙酮酸)和2.2%、4.3%(苯乙酸)。该方法已成功用于PKU患儿尿液中两种标志物的测定,且完全避免了尿液中尿酸对标志物测定的干扰,有望用于PKU患儿的早期无创诊断。展开更多
High performance capillary electrophoresis with amperometric detection was applied to the study on hydrolysis of adenosine monophosphate(AMP) in this article. Under the optimal conditions of electrophoresis(CTAB 0.2 m...High performance capillary electrophoresis with amperometric detection was applied to the study on hydrolysis of adenosine monophosphate(AMP) in this article. Under the optimal conditions of electrophoresis(CTAB 0.2 mmol/L+PBS 50 mmol/L, pH=8.2), the factors affecting the hydrolysis of AMP and mechanism of the hydrolysis were investigated. The results showed that the analytical method introduced was fast, convenient and efficient. [WT5HZ]展开更多
A glass capillary electrophoresis microchip with integrated amperometric detection cell equipped by replaceable micro-disk working electrode is described. The detection cell was fabricated as a buffer reservoir on the...A glass capillary electrophoresis microchip with integrated amperometric detection cell equipped by replaceable micro-disk working electrode is described. The detection cell was fabricated as a buffer reservoir on the microchip. A micro-electrode-guiding hole, which was in alignment with the exit of the separation channel, was drilled into the bottom edge of the chip. When a micro-disk copper working electrode, the outside diameter of which being matched with the inside diameter of the guiding hole, was inserted into the hole, passed through detection cell and finally got access to the exit of the separation channel, the electrode could be quasi-self-aligned to the channel exit and fixed inside the guiding hole without the help of a micro-positioner. Thus an integrated end-column amperometric detection system with replaceable disk working electrode was established on the microchip. Good reproducibility of the electrode alignment was demonstrated by a 4 1% RSD in the detection of sucrose after repeated(n=9) alignment of a micro-copper disk electrode. The developed michrochip was used for separation and detection of oligosaccharides.展开更多
文摘Using one step solid-phase extraction for urine pretreatment and a new sample focusing mode-dynamic pH junction injection for increasing sensitivity, a simple and sensitive method for the analysis of urinary 8OHdG by capillary electrophoresis with end-column amprometric detection has been developed. The limit of detection was 20 nmol/L(signal to noise ratio S/N=3). The urinary concentration of 8OHdG in nine healthy persons and 28 cancer patients was determined. It was found that urinary concentration of 8OHdG in cancer patients was significantly higher than that in healthy persons[(35.26±27.96) nmol/L) vs. (13.51±5.08) nmol/L, P<0.05], the result demonstrated that 8OHdG may be a good biomarker for measurement of oxidative DNA damage in cancer patients. Furthermore, the excretion levels of urinary 8OHdG from cancer patients receiving surgical therapy are investigated, and the results demonstrated that the smoking has a strong effect on 8OHdG content.
文摘首次采用毛细管电泳-安培检测法(CE-AD)同时测定了苯丙酮尿症(Phenylketonuria,PKU)患者尿液中的两种标志物:具有电活性的苯丙酮酸及非电活性的苯乙酸。考察了运行液添加剂、SDS浓度、工作电极电位等因素对分离和检测的影响。在优化实验条件下,两种标志物在15 m in内可实现基线分离。苯丙酮酸和苯乙酸的检出限(S/N=3)分别为0.50、0.92 mg/L。苯丙酮酸、苯乙酸浓度与峰高分别在3.0～1.0×103mg/L、2.0～5.0×103mg/L范围内呈良好线性,峰高与迁移时间的相对标准偏差分别为4.4%、3.2%(苯丙酮酸)和2.2%、4.3%(苯乙酸)。该方法已成功用于PKU患儿尿液中两种标志物的测定,且完全避免了尿液中尿酸对标志物测定的干扰,有望用于PKU患儿的早期无创诊断。
文摘High performance capillary electrophoresis with amperometric detection was applied to the study on hydrolysis of adenosine monophosphate(AMP) in this article. Under the optimal conditions of electrophoresis(CTAB 0.2 mmol/L+PBS 50 mmol/L, pH=8.2), the factors affecting the hydrolysis of AMP and mechanism of the hydrolysis were investigated. The results showed that the analytical method introduced was fast, convenient and efficient. [WT5HZ]
文摘A glass capillary electrophoresis microchip with integrated amperometric detection cell equipped by replaceable micro-disk working electrode is described. The detection cell was fabricated as a buffer reservoir on the microchip. A micro-electrode-guiding hole, which was in alignment with the exit of the separation channel, was drilled into the bottom edge of the chip. When a micro-disk copper working electrode, the outside diameter of which being matched with the inside diameter of the guiding hole, was inserted into the hole, passed through detection cell and finally got access to the exit of the separation channel, the electrode could be quasi-self-aligned to the channel exit and fixed inside the guiding hole without the help of a micro-positioner. Thus an integrated end-column amperometric detection system with replaceable disk working electrode was established on the microchip. Good reproducibility of the electrode alignment was demonstrated by a 4 1% RSD in the detection of sucrose after repeated(n=9) alignment of a micro-copper disk electrode. The developed michrochip was used for separation and detection of oligosaccharides.
