An effective and fast method for assay of micro-amounts of glucose was set up. A new technique for preparation of enzyme columns based on enzyme immobilization by sol-gel was investigated. Glucose oxidase(GOD) and hor...An effective and fast method for assay of micro-amounts of glucose was set up. A new technique for preparation of enzyme columns based on enzyme immobilization by sol-gel was investigated. Glucose oxidase(GOD) and horseradish peroxidase(HRP) mixed with SiO 2 nanoparticles and polyvinyl butyral(PVB) medium were immobilized on the surface of capillary tube, respectively. The experimental results show that nanoparticles can significantly enhance the catalytic activity of immobilized enzyme. Based on GOD column and HRP column, a liquid droplet sensor was developed for the determination of glucose solution. The effect of separated columns and mixed columns on the response of glucose sensor was investigated. The sensor showed a linear response in a range of 2-400 ng/mL with a detection limit of 0.3 ng/mL under the optimum conditions. The characteristics of the sensor including effect of flow-rate, pH and temperature were discussed.展开更多
文摘An effective and fast method for assay of micro-amounts of glucose was set up. A new technique for preparation of enzyme columns based on enzyme immobilization by sol-gel was investigated. Glucose oxidase(GOD) and horseradish peroxidase(HRP) mixed with SiO 2 nanoparticles and polyvinyl butyral(PVB) medium were immobilized on the surface of capillary tube, respectively. The experimental results show that nanoparticles can significantly enhance the catalytic activity of immobilized enzyme. Based on GOD column and HRP column, a liquid droplet sensor was developed for the determination of glucose solution. The effect of separated columns and mixed columns on the response of glucose sensor was investigated. The sensor showed a linear response in a range of 2-400 ng/mL with a detection limit of 0.3 ng/mL under the optimum conditions. The characteristics of the sensor including effect of flow-rate, pH and temperature were discussed.
基金financially supported by Applied Basic Research Program of Datong(No.2018147)Natural Science Foundation of Shanxi Province(No.201801D121035)+7 种基金Natural Science Foundation of Datong(No.201819)Scientific and Technological Innovation Programs of Higher Education Institutions in Shanxi(No.2019L0735,No.2020L0478,No.2020L0498)Cultivate Scientific Research Excellence Programs of Higher Education Institutions in Shanxi(No.2020KJ023)Key Scientific and Technological Projects of Datong City(No.2018014)Special Key Laboratory of Guizhou Province(No.[2018]004)the Science Foundation of the Science and Technology Department of Guizhou Province(No.[2014]17)Natural Science Research Project of the Education Department of Guizhou Province(No.[2015]386)Science and Technology of Sichuan Province(No.2016JY0096)。
