An excellent analytical tool for the separation and analysis of TCM(Traditional Chinese Medicine) by using HR ESI TOF MS(High Resolution Electrospray Ionization Time of flight Mass Spectrometry) was developed. A home ...An excellent analytical tool for the separation and analysis of TCM(Traditional Chinese Medicine) by using HR ESI TOF MS(High Resolution Electrospray Ionization Time of flight Mass Spectrometry) was developed. A home built HR ESI TOF MS was applied to detect fat soluble components in Danshen( Salvia miltiorrhiza Bage ) directly. Compared with the method of HPLC ESI MS(High Performance Liquid Chromatograph Electrospray ionization Mass Spectrometry) method, HR ESI TOF MS method is more accurate, quick, simple and reliable, so the method can become an effective tool for the quality control of TCM.展开更多
利用超高效液相色谱-高分辨四极杆飞行时间质谱对预混剂中的黄霉素5种组分进行鉴别分析。采用C18色谱柱分离,以乙腈-0.1%甲酸水溶液(含2 mmol/L乙酸铵)为流动相梯度洗脱,流速0.4 m L/min,柱温40℃;质谱条件为电喷雾离子源,检测方式为负...利用超高效液相色谱-高分辨四极杆飞行时间质谱对预混剂中的黄霉素5种组分进行鉴别分析。采用C18色谱柱分离,以乙腈-0.1%甲酸水溶液(含2 mmol/L乙酸铵)为流动相梯度洗脱,流速0.4 m L/min,柱温40℃;质谱条件为电喷雾离子源,检测方式为负离子全扫描模式,通过保留时间、精确分子量和二级质谱特征碎片完成对黄霉素的5种组分的鉴别。与高效液相色谱方法比较,本方法具有判断准确、检测快速的特点,具有很好的应用价值。展开更多
文摘An excellent analytical tool for the separation and analysis of TCM(Traditional Chinese Medicine) by using HR ESI TOF MS(High Resolution Electrospray Ionization Time of flight Mass Spectrometry) was developed. A home built HR ESI TOF MS was applied to detect fat soluble components in Danshen( Salvia miltiorrhiza Bage ) directly. Compared with the method of HPLC ESI MS(High Performance Liquid Chromatograph Electrospray ionization Mass Spectrometry) method, HR ESI TOF MS method is more accurate, quick, simple and reliable, so the method can become an effective tool for the quality control of TCM.
文摘利用超高效液相色谱-高分辨四极杆飞行时间质谱对预混剂中的黄霉素5种组分进行鉴别分析。采用C18色谱柱分离,以乙腈-0.1%甲酸水溶液(含2 mmol/L乙酸铵)为流动相梯度洗脱,流速0.4 m L/min,柱温40℃;质谱条件为电喷雾离子源,检测方式为负离子全扫描模式,通过保留时间、精确分子量和二级质谱特征碎片完成对黄霉素的5种组分的鉴别。与高效液相色谱方法比较,本方法具有判断准确、检测快速的特点,具有很好的应用价值。
