The concept of the imperfection sensitive region is given. The advanced stochastic imperfection method is used to research the imperfection sensitive region of single-layer latticed domes. Taking a K6 single-layer lat...The concept of the imperfection sensitive region is given. The advanced stochastic imperfection method is used to research the imperfection sensitive region of single-layer latticed domes. Taking a K6 single-layer latticed dome with a diameter of 50 m as an example, its imperfection sensitive region is made up of the first 12 kinds of joints. The influence of the imperfections of support joints on the stability of the K6 single-layer latticed dome is negligible. Influences of the joint imperfections of the main rib and the secondary rib on the structural stability are similar. The initial deviations of these joints all greatly lower the critical load of the dome. Results show that the method can analyze the structural imperfection sensitive region quantitatively and accurately.展开更多
To obtain high efficiency of cleavage of thrombin in fusion protein containing a ANP fusetl to Re f pep-tides,the linker sequence deslgned as VIAGR which was dlfferent from GVRGPR formerly used was stud-ied. Plasmld p...To obtain high efficiency of cleavage of thrombin in fusion protein containing a ANP fusetl to Re f pep-tides,the linker sequence deslgned as VIAGR which was dlfferent from GVRGPR formerly used was stud-ied. Plasmld pHL carrying the fuslon gene Ref-NT-ANP downstream from PL Proruc)ter was derived fromexpression vector pLY1 by inserting the fragment of NT-ANP lnto lt. The exPresslon of fuslon gene waslnduced at 420,and the interested proteln Ref-NT-ANP accumlllated as inclusion bodies was lsolated bygradient centrifuge and then dissolved in 7 mol/L guanidinehydrochloride(Gdn-HCl). After dilution,renat-uration and dialyzation, the cleavage of thrombin was examined using samples with 1. 1 mol/1, (;dn-HC1and samples free of Gdn-HCl resI)ective1y. I)igestion result showed that the novel-ad()Pted cleavage sequencewas highly sensitive to thrombin when the substrate dissOlved in 1. 1 mol/I, tidn HCl. The time needed for87%cleavage (the ratio of substrate to thrombin was 5O pg/u)was less than 24 hOurs. This sequence described here which was sPecifically recognized by thrombin might be broadly applied in other fusion sys-tems.展开更多
文摘The concept of the imperfection sensitive region is given. The advanced stochastic imperfection method is used to research the imperfection sensitive region of single-layer latticed domes. Taking a K6 single-layer latticed dome with a diameter of 50 m as an example, its imperfection sensitive region is made up of the first 12 kinds of joints. The influence of the imperfections of support joints on the stability of the K6 single-layer latticed dome is negligible. Influences of the joint imperfections of the main rib and the secondary rib on the structural stability are similar. The initial deviations of these joints all greatly lower the critical load of the dome. Results show that the method can analyze the structural imperfection sensitive region quantitatively and accurately.
文摘To obtain high efficiency of cleavage of thrombin in fusion protein containing a ANP fusetl to Re f pep-tides,the linker sequence deslgned as VIAGR which was dlfferent from GVRGPR formerly used was stud-ied. Plasmld pHL carrying the fuslon gene Ref-NT-ANP downstream from PL Proruc)ter was derived fromexpression vector pLY1 by inserting the fragment of NT-ANP lnto lt. The exPresslon of fuslon gene waslnduced at 420,and the interested proteln Ref-NT-ANP accumlllated as inclusion bodies was lsolated bygradient centrifuge and then dissolved in 7 mol/L guanidinehydrochloride(Gdn-HCl). After dilution,renat-uration and dialyzation, the cleavage of thrombin was examined using samples with 1. 1 mol/1, (;dn-HC1and samples free of Gdn-HCl resI)ective1y. I)igestion result showed that the novel-ad()Pted cleavage sequencewas highly sensitive to thrombin when the substrate dissOlved in 1. 1 mol/I, tidn HCl. The time needed for87%cleavage (the ratio of substrate to thrombin was 5O pg/u)was less than 24 hOurs. This sequence described here which was sPecifically recognized by thrombin might be broadly applied in other fusion sys-tems.
