The cDNA of human Interleukin\|18 was obtained by RT\|PCR from the mRNA of human peripheral blood cells.The DNA sequencing was performed and the DNA was cloned into expression vector pBV220.The overexpression was perf...The cDNA of human Interleukin\|18 was obtained by RT\|PCR from the mRNA of human peripheral blood cells.The DNA sequencing was performed and the DNA was cloned into expression vector pBV220.The overexpression was performed in E.coli HB101.The expressive product was purified through Sephadex G100 and identified by N\|terminal amino acid sequencing and Western blot.展开更多
白细胞介素-2(IL-2)是一种T细胞生长因子,具有许多生物活性,例如诱导细胞毒性T细胞,激活自然杀伤细胞,诱生LAK(淋巴因子激活的杀伤细胞),提高IFN-γ产量[1],激活B细胞而参与机体的体液免疫.IL-2是由辅助型T细胞(helper T cell)Th1细胞...白细胞介素-2(IL-2)是一种T细胞生长因子,具有许多生物活性,例如诱导细胞毒性T细胞,激活自然杀伤细胞,诱生LAK(淋巴因子激活的杀伤细胞),提高IFN-γ产量[1],激活B细胞而参与机体的体液免疫.IL-2是由辅助型T细胞(helper T cell)Th1细胞分泌的,通过激活包括T细胞,NK细胞和LAK细胞的受体而介导细胞毒性反应,从而提高细胞毒性防御[2].展开更多
文摘The cDNA of human Interleukin\|18 was obtained by RT\|PCR from the mRNA of human peripheral blood cells.The DNA sequencing was performed and the DNA was cloned into expression vector pBV220.The overexpression was performed in E.coli HB101.The expressive product was purified through Sephadex G100 and identified by N\|terminal amino acid sequencing and Western blot.
文摘白细胞介素-2(IL-2)是一种T细胞生长因子,具有许多生物活性,例如诱导细胞毒性T细胞,激活自然杀伤细胞,诱生LAK(淋巴因子激活的杀伤细胞),提高IFN-γ产量[1],激活B细胞而参与机体的体液免疫.IL-2是由辅助型T细胞(helper T cell)Th1细胞分泌的,通过激活包括T细胞,NK细胞和LAK细胞的受体而介导细胞毒性反应,从而提高细胞毒性防御[2].
