A new method for preliminary screening of anticancer drugs by Hadamard transform microscopic fluorescence image analysis was proposed. Rat liver 2c hepatocyte was usedas the standard cell and acridine orange (AO) was ...A new method for preliminary screening of anticancer drugs by Hadamard transform microscopic fluorescence image analysis was proposed. Rat liver 2c hepatocyte was usedas the standard cell and acridine orange (AO) was employed to trace the interaction of drugand cellular DNA. The results for five anticancer drugs, vincristine, cyclophosphamide,mustine, cis-platin and mitomycin-C are in accordance with the mechanism of the drugs binding to cellular DNA and this indicates that this method is suitable for screening cell cyclenonspecific anticancer drugs.展开更多
Telomerase, a ribonucleoprotein enzyme, has been found in immortalized but not in most somatic adult human tissues, and thus emerged as a novel target for cancer chemotherapy. Recently it has been found that telomeras...Telomerase, a ribonucleoprotein enzyme, has been found in immortalized but not in most somatic adult human tissues, and thus emerged as a novel target for cancer chemotherapy. Recently it has been found that telomerase is a fruitful target for oncologic drug development. A new method for screening antitumor agents by using telomerase as a target has been established according to the phenomena that the enzyme activity can be affected by some types of antitumor agents or chemicals. The telomerase was extracted from HeLa cells. The telomeric repeat amplification protocol(TRAP) was used to measure enzyme activity. Telomerase activity can be inhibited by 4 kinds of chemical compounds.展开更多
文摘A new method for preliminary screening of anticancer drugs by Hadamard transform microscopic fluorescence image analysis was proposed. Rat liver 2c hepatocyte was usedas the standard cell and acridine orange (AO) was employed to trace the interaction of drugand cellular DNA. The results for five anticancer drugs, vincristine, cyclophosphamide,mustine, cis-platin and mitomycin-C are in accordance with the mechanism of the drugs binding to cellular DNA and this indicates that this method is suitable for screening cell cyclenonspecific anticancer drugs.
文摘Telomerase, a ribonucleoprotein enzyme, has been found in immortalized but not in most somatic adult human tissues, and thus emerged as a novel target for cancer chemotherapy. Recently it has been found that telomerase is a fruitful target for oncologic drug development. A new method for screening antitumor agents by using telomerase as a target has been established according to the phenomena that the enzyme activity can be affected by some types of antitumor agents or chemicals. The telomerase was extracted from HeLa cells. The telomeric repeat amplification protocol(TRAP) was used to measure enzyme activity. Telomerase activity can be inhibited by 4 kinds of chemical compounds.
