Some natural products were extracted from Sophora flavesence Ait., Prenanthes purpurea L. Saussurea japonica DC., Aster tataricus L., Rubia cordifo lia L., Mosta chinensis Maxim. and Orostachy fimbriatus Berg. Their a...Some natural products were extracted from Sophora flavesence Ait., Prenanthes purpurea L. Saussurea japonica DC., Aster tataricus L., Rubia cordifo lia L., Mosta chinensis Maxim. and Orostachy fimbriatus Berg. Their anti-phytovirus and anti-fungus activities have been tested, some of these products are hopeful to be selected as lead compounds for developing new pesticides.展开更多
以[^(14)C]碳酸钡为放射性同位素原料,通过格氏反应、Curtius重排、亲核加成、硫代及关环等7步反应,制备了同位素碳-14标记的毒氟磷粗品,经反相高效液相色谱(RP-HPLC)纯化获得标记物纯品^(14)C-毒氟磷(N-[2-(4-甲基苯并[2-^(14)C]噻唑基...以[^(14)C]碳酸钡为放射性同位素原料,通过格氏反应、Curtius重排、亲核加成、硫代及关环等7步反应,制备了同位素碳-14标记的毒氟磷粗品,经反相高效液相色谱(RP-HPLC)纯化获得标记物纯品^(14)C-毒氟磷(N-[2-(4-甲基苯并[2-^(14)C]噻唑基)]-2-氨基-2-氟代苯基-O,O-二乙基甲基膦酸酯,38.3 m Ci)。7步反应的化学收率/放化收率为10%。其结构经核磁共振氢谱、质谱和放射性高效液相色谱(HPLC-FSA)分析确认。放射性薄层成像分析(TLC-IIA)、高效液相色谱-液体闪烁测量联用分析(HPLC-LSC)、高效液相色谱-流动液体闪烁测量/二极管阵列检测器/质谱联用分析(HPLC-FSA/PDA/MS)和LSC分析表明,^(14)C-毒氟磷的放化纯度和化学纯度均大于98%,比活度为58.0 m Ci/mmol,可作为放射性示踪剂,用于毒氟磷的代谢和环境行为等研究。展开更多
文摘Some natural products were extracted from Sophora flavesence Ait., Prenanthes purpurea L. Saussurea japonica DC., Aster tataricus L., Rubia cordifo lia L., Mosta chinensis Maxim. and Orostachy fimbriatus Berg. Their anti-phytovirus and anti-fungus activities have been tested, some of these products are hopeful to be selected as lead compounds for developing new pesticides.
文摘以[^(14)C]碳酸钡为放射性同位素原料,通过格氏反应、Curtius重排、亲核加成、硫代及关环等7步反应,制备了同位素碳-14标记的毒氟磷粗品,经反相高效液相色谱(RP-HPLC)纯化获得标记物纯品^(14)C-毒氟磷(N-[2-(4-甲基苯并[2-^(14)C]噻唑基)]-2-氨基-2-氟代苯基-O,O-二乙基甲基膦酸酯,38.3 m Ci)。7步反应的化学收率/放化收率为10%。其结构经核磁共振氢谱、质谱和放射性高效液相色谱(HPLC-FSA)分析确认。放射性薄层成像分析(TLC-IIA)、高效液相色谱-液体闪烁测量联用分析(HPLC-LSC)、高效液相色谱-流动液体闪烁测量/二极管阵列检测器/质谱联用分析(HPLC-FSA/PDA/MS)和LSC分析表明,^(14)C-毒氟磷的放化纯度和化学纯度均大于98%,比活度为58.0 m Ci/mmol,可作为放射性示踪剂,用于毒氟磷的代谢和环境行为等研究。
