利用42个优质小麦(Triticum aestivum)品种籽粒发芽指数对6个小麦穗发芽抗性相关基因[Tamyb10、TaDFR(Dihydroflavone reductase)、TaVp-1(Viviparous‑1)、TaSdr(Seed dormancy)、TaPM19-A1(Plasma membrane 19‑A1)、TaMFT(Mother of FT...利用42个优质小麦(Triticum aestivum)品种籽粒发芽指数对6个小麦穗发芽抗性相关基因[Tamyb10、TaDFR(Dihydroflavone reductase)、TaVp-1(Viviparous‑1)、TaSdr(Seed dormancy)、TaPM19-A1(Plasma membrane 19‑A1)、TaMFT(Mother of FT and TFL1)]的分子标记的有效性进行验证,以期为抗穗发芽优质小麦品种的筛选及选育奠定基础。结果表明,小麦穗发芽抗性相关基因Tamyb10、TaDFR、TaVp-1、TaSdr、TaPM19-A1、TaMFT在42个优质小麦品种中均检测到2种等位变异,优异等位基因所占比例存在明显差异,介于4.8%~78.6%,未发现TaVp-1的TaVp-1 Bb基因型。等位基因类型与籽粒发芽指数相关性分析表明,标记myb10D、MFT-3A和MFT-A2与优质小麦穗发芽抗性极显著相关,而标记DFR-B、Vp1B3、Sdr2A、Sdr2B和PM19-A1与优质小麦穗发芽抗性相关性不显著。STS标记myb10D可用于红粒优质小麦的穗发芽抗性筛选,CAPS标记MFT-3A和STS标记MFT-A2可用于白粒优质小麦的穗发芽抗性筛选,TaMFT可能在优质小麦抗穗发芽机制中发挥着重要作用。展开更多
A total of 144 F2 individuals were obtained from the crossing between 1047 (susceptible) and A21 (resistant). Two RAPD markers were screened out in 200 random primers using BSA(Bulked Segregant Analysis). Two RAPD mar...A total of 144 F2 individuals were obtained from the crossing between 1047 (susceptible) and A21 (resistant). Two RAPD markers were screened out in 200 random primers using BSA(Bulked Segregant Analysis). Two RAPD markers, designated as AG13/2000 and U16/660, were 7.7 cM and 8.38 cM apart from the TuMV resistant gene, respectively. The two RAPD fragments were converted to SCAR markers. SCAR markers were confined in germplasm.展开更多
文摘利用42个优质小麦(Triticum aestivum)品种籽粒发芽指数对6个小麦穗发芽抗性相关基因[Tamyb10、TaDFR(Dihydroflavone reductase)、TaVp-1(Viviparous‑1)、TaSdr(Seed dormancy)、TaPM19-A1(Plasma membrane 19‑A1)、TaMFT(Mother of FT and TFL1)]的分子标记的有效性进行验证,以期为抗穗发芽优质小麦品种的筛选及选育奠定基础。结果表明,小麦穗发芽抗性相关基因Tamyb10、TaDFR、TaVp-1、TaSdr、TaPM19-A1、TaMFT在42个优质小麦品种中均检测到2种等位变异,优异等位基因所占比例存在明显差异,介于4.8%~78.6%,未发现TaVp-1的TaVp-1 Bb基因型。等位基因类型与籽粒发芽指数相关性分析表明,标记myb10D、MFT-3A和MFT-A2与优质小麦穗发芽抗性极显著相关,而标记DFR-B、Vp1B3、Sdr2A、Sdr2B和PM19-A1与优质小麦穗发芽抗性相关性不显著。STS标记myb10D可用于红粒优质小麦的穗发芽抗性筛选,CAPS标记MFT-3A和STS标记MFT-A2可用于白粒优质小麦的穗发芽抗性筛选,TaMFT可能在优质小麦抗穗发芽机制中发挥着重要作用。
文摘A total of 144 F2 individuals were obtained from the crossing between 1047 (susceptible) and A21 (resistant). Two RAPD markers were screened out in 200 random primers using BSA(Bulked Segregant Analysis). Two RAPD markers, designated as AG13/2000 and U16/660, were 7.7 cM and 8.38 cM apart from the TuMV resistant gene, respectively. The two RAPD fragments were converted to SCAR markers. SCAR markers were confined in germplasm.
