Taking Small Tail Han Sheeps ear margin as the study materials, we succeeded in establishing a fibroblast cell bank which content of samples was 52 by the method of explant culture directly and cryopreservation techni...Taking Small Tail Han Sheeps ear margin as the study materials, we succeeded in establishing a fibroblast cell bank which content of samples was 52 by the method of explant culture directly and cryopreservation technique. The biological analysis results showed that population doubling time (PDT) of cells was approximately 24 h, diploid account for 90%～96% in chromosome. Confirmed by isoenzyme analysis,this cell line had no cross-contamination.The test results of the bacterium, fungi, virus, mycoplasma were negative. The transfection rate was high, and the extro-gene can be effectively expressed. Every indexes of the cell bank met all the standard quality controls of ATCC. It had not only preserved the germplasm resources of this important Small Tail Han Sheep on the cell level but also provided valuable material for the research of genome, postgenome and somacloning. Meanwhile, the setting-up of this technological platform will offer technology and theory for storing the cell genetic resources of other animals and poultry.展开更多
为探寻黑豆皮花色苷(anthocyanins from black soybean seed coat,ABSC)抗禽白血病病毒A亚群(subgroup A avian leukosis virus,ALV-A)的活性效果。本文以ABSC粉为原料,采用高效液相色谱仪与质谱仪联机(HPLC-MS)的方法鉴定了其花色苷主...为探寻黑豆皮花色苷(anthocyanins from black soybean seed coat,ABSC)抗禽白血病病毒A亚群(subgroup A avian leukosis virus,ALV-A)的活性效果。本文以ABSC粉为原料,采用高效液相色谱仪与质谱仪联机(HPLC-MS)的方法鉴定了其花色苷主要活性成分,随后采用体外实验,应用MTT法和观察细胞形态法检测了ABSC对鸡成纤维细胞系(DF-1)的细胞毒性,建立细胞模型,研究加入ABSC后对ALV-A的预防和治疗作用。结果表明:黑豆皮花色苷粉中含四种花色苷成分,其中主要为矢车菊色素-3-葡萄糖苷;ABSC对DF-1无细胞毒性的最大相对安全质量浓度为20μg/mL;ABSC在安全浓度范围内能抑制ALV-A的增殖,且抑制程度成剂量关系。结论,ABSC质量浓度为12μg/mL能够显著抑制ALV-A诱导的DF-1细胞形态变化,降低凋亡。展开更多
文摘Taking Small Tail Han Sheeps ear margin as the study materials, we succeeded in establishing a fibroblast cell bank which content of samples was 52 by the method of explant culture directly and cryopreservation technique. The biological analysis results showed that population doubling time (PDT) of cells was approximately 24 h, diploid account for 90%～96% in chromosome. Confirmed by isoenzyme analysis,this cell line had no cross-contamination.The test results of the bacterium, fungi, virus, mycoplasma were negative. The transfection rate was high, and the extro-gene can be effectively expressed. Every indexes of the cell bank met all the standard quality controls of ATCC. It had not only preserved the germplasm resources of this important Small Tail Han Sheep on the cell level but also provided valuable material for the research of genome, postgenome and somacloning. Meanwhile, the setting-up of this technological platform will offer technology and theory for storing the cell genetic resources of other animals and poultry.
文摘为探寻黑豆皮花色苷(anthocyanins from black soybean seed coat,ABSC)抗禽白血病病毒A亚群(subgroup A avian leukosis virus,ALV-A)的活性效果。本文以ABSC粉为原料,采用高效液相色谱仪与质谱仪联机(HPLC-MS)的方法鉴定了其花色苷主要活性成分,随后采用体外实验,应用MTT法和观察细胞形态法检测了ABSC对鸡成纤维细胞系(DF-1)的细胞毒性,建立细胞模型,研究加入ABSC后对ALV-A的预防和治疗作用。结果表明:黑豆皮花色苷粉中含四种花色苷成分,其中主要为矢车菊色素-3-葡萄糖苷;ABSC对DF-1无细胞毒性的最大相对安全质量浓度为20μg/mL;ABSC在安全浓度范围内能抑制ALV-A的增殖,且抑制程度成剂量关系。结论,ABSC质量浓度为12μg/mL能够显著抑制ALV-A诱导的DF-1细胞形态变化,降低凋亡。
