Studying the skin care efficacy of recombinant humanized collagen based on in vitro level.The stability of the recombinant humanized collagen was first analyzed by treating at different temperatures,then its skincare ...Studying the skin care efficacy of recombinant humanized collagen based on in vitro level.The stability of the recombinant humanized collagen was first analyzed by treating at different temperatures,then its skincare efficacy based on in vitro level was evaluated by detecting the inhibition rate of elastase,the inhibition rate of collagenase,the protein content of type I collagen in human fibroblasts,the inhibition of reactive oxygen species(ROS)with human keratinocytes,and the effects of the recombinant humanized collagen on the expression of hyaluronic acid(HA),filaggrin(FLG)and transglutaminase 1(TGM1)in keratinocytes.The results showed that recombinant humanized collagen was able to maintain stability at temperatures below 70℃.With regard to its skincare efficacy,recombinant humanized collagen could inhibit elastase and collagenase activities and promote the increase of type I collagen content in human fibroblasts.It also showed good inhibition of ROS in keratinocytes in vitro and could increase the expression of HA,FLG,and TGM1 in keratinocytes.In short,the recombinant humanized collagen exhibited a favourable skin care effect in vitro level.This study proved that it has potential firming,anti-wrinkle,moisturizing,and repairing efficacy,and is a valuable cosmetic raw material.展开更多
Based on the basic geological features of Yixingzhai gold deposit, the geological features, classification and relation to mineralization of breccia pipes were discussed by surveying the xenoliths and breccia's mi...Based on the basic geological features of Yixingzhai gold deposit, the geological features, classification and relation to mineralization of breccia pipes were discussed by surveying the xenoliths and breccia's mineral composition, structure and construction, typical mineralization alteration phenomenon on field and microscopic anatomy in breccia pipes. And the ore-controlling mechanism and the formation mechanism of the blasting breccia pipes were investigated. The main conclusion shows that Hewan and Nanmenshan breccia pipes nearby the NW-striking deep fault are shaped earlier, belonging to the acidic siliceous ingredient production of ultra-hypabyssal magmatic in the late magma stage; Tietangdong and Nanmenshan breccia pipes that are shaped latter are the ultra-hypabyssal auriferous fluid production composed mainly of the skarn in the hydrothermal stage after the magma period. Both root in the unified deep magmatic chamber, belonging to differentiation derivatives at different stages in the deep magmatic chamber.展开更多
文摘Studying the skin care efficacy of recombinant humanized collagen based on in vitro level.The stability of the recombinant humanized collagen was first analyzed by treating at different temperatures,then its skincare efficacy based on in vitro level was evaluated by detecting the inhibition rate of elastase,the inhibition rate of collagenase,the protein content of type I collagen in human fibroblasts,the inhibition of reactive oxygen species(ROS)with human keratinocytes,and the effects of the recombinant humanized collagen on the expression of hyaluronic acid(HA),filaggrin(FLG)and transglutaminase 1(TGM1)in keratinocytes.The results showed that recombinant humanized collagen was able to maintain stability at temperatures below 70℃.With regard to its skincare efficacy,recombinant humanized collagen could inhibit elastase and collagenase activities and promote the increase of type I collagen content in human fibroblasts.It also showed good inhibition of ROS in keratinocytes in vitro and could increase the expression of HA,FLG,and TGM1 in keratinocytes.In short,the recombinant humanized collagen exhibited a favourable skin care effect in vitro level.This study proved that it has potential firming,anti-wrinkle,moisturizing,and repairing efficacy,and is a valuable cosmetic raw material.
基金Project(2007CB416608) supported by the Major State Basic Research Development Program of ChinaProject(2006BAB01B07) supported by the National Scientific Project for Tackcling Key Problems
文摘Based on the basic geological features of Yixingzhai gold deposit, the geological features, classification and relation to mineralization of breccia pipes were discussed by surveying the xenoliths and breccia's mineral composition, structure and construction, typical mineralization alteration phenomenon on field and microscopic anatomy in breccia pipes. And the ore-controlling mechanism and the formation mechanism of the blasting breccia pipes were investigated. The main conclusion shows that Hewan and Nanmenshan breccia pipes nearby the NW-striking deep fault are shaped earlier, belonging to the acidic siliceous ingredient production of ultra-hypabyssal magmatic in the late magma stage; Tietangdong and Nanmenshan breccia pipes that are shaped latter are the ultra-hypabyssal auriferous fluid production composed mainly of the skarn in the hydrothermal stage after the magma period. Both root in the unified deep magmatic chamber, belonging to differentiation derivatives at different stages in the deep magmatic chamber.
