目的:探讨力竭运动后不同时相心脏窦房结、房室结和浦肯野氏纤维炎性因子细胞间粘附分子-1(ICAM-1)基因和蛋白水平的表达特点,为运动性心肌损伤与心律失常发生机制的阐明提供实验依据。方法:100只健康成年雄性SD大鼠随机分为10组,每组10...目的:探讨力竭运动后不同时相心脏窦房结、房室结和浦肯野氏纤维炎性因子细胞间粘附分子-1(ICAM-1)基因和蛋白水平的表达特点,为运动性心肌损伤与心律失常发生机制的阐明提供实验依据。方法:100只健康成年雄性SD大鼠随机分为10组,每组10只,分别为一次力竭运动组(4组,n=40)、反复力竭运动组(4组,n=40)、一次力竭安静对照组(n=10)和反复力竭安静对照组(n=10)。分别于力竭运动后0、4、12及24小时取材,进行免疫荧光组化及实时荧光定量PCR分析。应用激光显微切割技术定位并收集窦房结、房室结和浦肯野氏纤维细胞,研究细胞炎性因子ICAM-1的m RNA和蛋白表达的变化。结果:一次力竭运动后心脏窦房结ICAM-1 m RNA和蛋白表达在运动后12小时达峰值,显著高于对照组(P<0.05),浦肯野氏纤维在运动后24小时达峰值,显著高于对照组(P<0.01)。反复力竭运动后心脏窦房结ICAM-1 m RNA和蛋白表达在运动后即刻和12小时出现两次峰值,显著高于对照组(P<0.01),浦肯野氏纤维ICAM-1蛋白表达12小时达峰值,显著高于对照组(P<0.01)。结论:力竭运动后心脏传导系统不同部位ICAM-1 m RNA和蛋白表达的时相规律各异,但心脏窦房结炎性因子增高幅度较大,是运动性心肌损伤与心律失常的机制之一。展开更多
This paper reviews the ageing changes of the human cardiac conduction system according to the ever published literature. In ageing process, the cardiac conduction system shows typical physiological changes: (1) differ...This paper reviews the ageing changes of the human cardiac conduction system according to the ever published literature. In ageing process, the cardiac conduction system shows typical physiological changes: (1) differentiation, development and maturation in fetal and postnatal period, (2)reduction of the specific conduction cells and the changes of the whole node volumes. (3) fibrosis and fat infiltration. It is stressed that while explaning the causes of sudden death with the knowledge of cardiac conduction system diseases, the forensic pathologist ahould be able to distinguish the pathological changes from that of the normalagemg.展开更多
文摘目的:探讨力竭运动后不同时相心脏窦房结、房室结和浦肯野氏纤维炎性因子细胞间粘附分子-1(ICAM-1)基因和蛋白水平的表达特点,为运动性心肌损伤与心律失常发生机制的阐明提供实验依据。方法:100只健康成年雄性SD大鼠随机分为10组,每组10只,分别为一次力竭运动组(4组,n=40)、反复力竭运动组(4组,n=40)、一次力竭安静对照组(n=10)和反复力竭安静对照组(n=10)。分别于力竭运动后0、4、12及24小时取材,进行免疫荧光组化及实时荧光定量PCR分析。应用激光显微切割技术定位并收集窦房结、房室结和浦肯野氏纤维细胞,研究细胞炎性因子ICAM-1的m RNA和蛋白表达的变化。结果:一次力竭运动后心脏窦房结ICAM-1 m RNA和蛋白表达在运动后12小时达峰值,显著高于对照组(P<0.05),浦肯野氏纤维在运动后24小时达峰值,显著高于对照组(P<0.01)。反复力竭运动后心脏窦房结ICAM-1 m RNA和蛋白表达在运动后即刻和12小时出现两次峰值,显著高于对照组(P<0.01),浦肯野氏纤维ICAM-1蛋白表达12小时达峰值,显著高于对照组(P<0.01)。结论:力竭运动后心脏传导系统不同部位ICAM-1 m RNA和蛋白表达的时相规律各异,但心脏窦房结炎性因子增高幅度较大,是运动性心肌损伤与心律失常的机制之一。
文摘This paper reviews the ageing changes of the human cardiac conduction system according to the ever published literature. In ageing process, the cardiac conduction system shows typical physiological changes: (1) differentiation, development and maturation in fetal and postnatal period, (2)reduction of the specific conduction cells and the changes of the whole node volumes. (3) fibrosis and fat infiltration. It is stressed that while explaning the causes of sudden death with the knowledge of cardiac conduction system diseases, the forensic pathologist ahould be able to distinguish the pathological changes from that of the normalagemg.
