The studies on leave protein of Betula platyphylla seedlings by the methods of SDS PAGE electrophoresis and dimensional gel electrophoresis was concerned. The result indicated that some new proteins such as 29kD pI4 3...The studies on leave protein of Betula platyphylla seedlings by the methods of SDS PAGE electrophoresis and dimensional gel electrophoresis was concerned. The result indicated that some new proteins such as 29kD pI4 3, 29kD pI4 2, 14kD pI5 3 and 11 5kD pI5 16 protein point were induced by soil drought stress, and some protein disappeared, for instance four protein between 12 4～11 5kD pI5 8～6 44 in seedlings leaves of B. platyphylla during soil drought stress.展开更多
对蛋白质组学中的双向聚丙烯酰胺凝胶电泳(two-dimensional gel electrophoresis,简称2-DE)、相对和绝对定量同位素标记(isobaric tags for relative and absolute quantification,简称iTRAQ)2种技术进行比较,以期为石榴的蛋白质组学研...对蛋白质组学中的双向聚丙烯酰胺凝胶电泳(two-dimensional gel electrophoresis,简称2-DE)、相对和绝对定量同位素标记(isobaric tags for relative and absolute quantification,简称iTRAQ)2种技术进行比较,以期为石榴的蛋白质组学研究提供选择参考。取花后120 d的三白石榴籽粒为材料,分别用双向电泳、iTRAQ 2种技术检测三白石榴籽粒蛋白。结果表明,利用2-DE技术检测到890种蛋白;通过质谱鉴定出5种蛋白;利用iTRAQ技术得到1 940种蛋白,其中分子量在10 ku以下的有16种,200 ku以上的有19种,pH值大于11的有11种,说明iTRAQ技术在鉴定大分子、小分子蛋白方面具有优势。由以上结果可知,iTRAQ技术比2-DE技术能发现更多数量、更多种类的蛋白,而且iTRAQ技术可以通过信息检索了解到被检索肽段的真实身份,该技术的出现,使得对同种蛋白质表达变化的鉴定更为准确。展开更多
文摘The studies on leave protein of Betula platyphylla seedlings by the methods of SDS PAGE electrophoresis and dimensional gel electrophoresis was concerned. The result indicated that some new proteins such as 29kD pI4 3, 29kD pI4 2, 14kD pI5 3 and 11 5kD pI5 16 protein point were induced by soil drought stress, and some protein disappeared, for instance four protein between 12 4～11 5kD pI5 8～6 44 in seedlings leaves of B. platyphylla during soil drought stress.
文摘对蛋白质组学中的双向聚丙烯酰胺凝胶电泳(two-dimensional gel electrophoresis,简称2-DE)、相对和绝对定量同位素标记(isobaric tags for relative and absolute quantification,简称iTRAQ)2种技术进行比较,以期为石榴的蛋白质组学研究提供选择参考。取花后120 d的三白石榴籽粒为材料,分别用双向电泳、iTRAQ 2种技术检测三白石榴籽粒蛋白。结果表明,利用2-DE技术检测到890种蛋白;通过质谱鉴定出5种蛋白;利用iTRAQ技术得到1 940种蛋白,其中分子量在10 ku以下的有16种,200 ku以上的有19种,pH值大于11的有11种,说明iTRAQ技术在鉴定大分子、小分子蛋白方面具有优势。由以上结果可知,iTRAQ技术比2-DE技术能发现更多数量、更多种类的蛋白,而且iTRAQ技术可以通过信息检索了解到被检索肽段的真实身份,该技术的出现,使得对同种蛋白质表达变化的鉴定更为准确。
