以壳寡糖为诱导物处理烟草植株,利用高效液相色谱 电化学(库仑电极)阵列检测技术检测不同处理时间植物体内内源激素的变化情况及其他小分子物质的差异显示。所用色谱柱为HypersilBDSC18柱(4.6mmi d ×200mm,5μm),八通道电化学阵列...以壳寡糖为诱导物处理烟草植株,利用高效液相色谱 电化学(库仑电极)阵列检测技术检测不同处理时间植物体内内源激素的变化情况及其他小分子物质的差异显示。所用色谱柱为HypersilBDSC18柱(4.6mmi d ×200mm,5μm),八通道电化学阵列检测器(八个电极)的检测电势依次为-200,300,400,500,600,700,800,850mV。结果表明,茉莉酸(JA)和赤霉酸(GA)含量在用壳寡糖处理6h时达到高峰,吲哚乙酸(IAA)含量在处理8h时达到高峰。处理后烟草植株产生的其他一些未知的小分子物质尚有待定性和进一步考察其变化规律。展开更多
A normal phase high performance chromatographic method with evaporative light scattering detector(ELSD) and diode array detector(DAD), using n hexane/methanol/ethyl acetate(volume ratio 6∶3∶2 ) as the mobile phase, ...A normal phase high performance chromatographic method with evaporative light scattering detector(ELSD) and diode array detector(DAD), using n hexane/methanol/ethyl acetate(volume ratio 6∶3∶2 ) as the mobile phase, was established for the direct determination of active constituents gastrodin(GA) in the ethanol extracts of Gastrodia elata Blume . An silica gel column Zorbax RX SIL\[4 6 mm (\{i.d.\})×25 cm, 5 μm\] was employed. Regression equations revealed the linear relationships( r =0 998 and 0 999) between the peak areas of GA measured by ELSD and DAD and its concentration. The recoveries of GA measured by ELSD and DAD are 104 22% and 97 65%, respectively. The contents of GA measured by ELSD and DAD are 13 9 mg/g(2 79% RSD) and 12 1 mg/g(2 99% RSD) respectively in Gastrodia elata Blume . The detection limits of ELSD and DAD( S/N =3) are 3 mg/L and 1 mg/L respectively. This method is simple, selective and sensitive.展开更多
文摘以壳寡糖为诱导物处理烟草植株,利用高效液相色谱 电化学(库仑电极)阵列检测技术检测不同处理时间植物体内内源激素的变化情况及其他小分子物质的差异显示。所用色谱柱为HypersilBDSC18柱(4.6mmi d ×200mm,5μm),八通道电化学阵列检测器(八个电极)的检测电势依次为-200,300,400,500,600,700,800,850mV。结果表明,茉莉酸(JA)和赤霉酸(GA)含量在用壳寡糖处理6h时达到高峰,吲哚乙酸(IAA)含量在处理8h时达到高峰。处理后烟草植株产生的其他一些未知的小分子物质尚有待定性和进一步考察其变化规律。
文摘A normal phase high performance chromatographic method with evaporative light scattering detector(ELSD) and diode array detector(DAD), using n hexane/methanol/ethyl acetate(volume ratio 6∶3∶2 ) as the mobile phase, was established for the direct determination of active constituents gastrodin(GA) in the ethanol extracts of Gastrodia elata Blume . An silica gel column Zorbax RX SIL\[4 6 mm (\{i.d.\})×25 cm, 5 μm\] was employed. Regression equations revealed the linear relationships( r =0 998 and 0 999) between the peak areas of GA measured by ELSD and DAD and its concentration. The recoveries of GA measured by ELSD and DAD are 104 22% and 97 65%, respectively. The contents of GA measured by ELSD and DAD are 13 9 mg/g(2 79% RSD) and 12 1 mg/g(2 99% RSD) respectively in Gastrodia elata Blume . The detection limits of ELSD and DAD( S/N =3) are 3 mg/L and 1 mg/L respectively. This method is simple, selective and sensitive.
