胃癌是我国的主要恶性肿瘤之一,其发病率及死亡率在恶性肿瘤中居于首位,严重影响人民的身体健康。近年来,虽然对胃癌的基础理论和临床作了大量的研究,但对胃癌的病因、生物学行为仍处在初步探索阶段,对早期胃癌的诊断还缺少客观化定量指...胃癌是我国的主要恶性肿瘤之一,其发病率及死亡率在恶性肿瘤中居于首位,严重影响人民的身体健康。近年来,虽然对胃癌的基础理论和临床作了大量的研究,但对胃癌的病因、生物学行为仍处在初步探索阶段,对早期胃癌的诊断还缺少客观化定量指标,治疗方法更是匮乏。因此,迫切需要对胃癌作更深入的研究。染色体在数量和结构上的畸变,是公认的一种癌细胞特征。DNA 是染色体的主要物质基础。正常胃粘膜细胞 DNA 含量较恒定,其倍体类型主要表现为二倍体。但由正常细胞经过转化细胞,直至变为癌细胞时。展开更多
Objective:To establish a sensitized canine model for kidney transplantation.Methods:12 male dogs were averagely grouped as donors and recipients. A small number of donor canine lymphocytes was infused into different a...Objective:To establish a sensitized canine model for kidney transplantation.Methods:12 male dogs were averagely grouped as donors and recipients. A small number of donor canine lymphocytes was infused into different anatomic locations of a paired canine recipient for each time and which was repeated weekly. Specific immune sensitization was monitored by means of Complement Dependent Cytotoxicity (CDC) and Mixed Lymphocyte Culture (MLC) test. When CDC test conversed to be positive and MLC test showed a significant proliferation of reactive lymphocytes of canine recipients, the right kidneys of the paired dogs were excised and transplanted to each other concurrently. Injury of renal allograft function was scheduled determined by ECT dynamic kidney photography and pathologic investigation. Results:CDC test usually conversed to be positive and reactive lymphocytes of canine recipients were also observed to be proliferated significantly in MLC test after 3 to 4 times of canine donor lymphocyte infusions. Renal allograft function deterioration occurred 4 d post-operatively in 4 of 6 canine recipients, in contrast to none in control dogs. Pathologic changes suggested antibody-mediated rejection (delayed) or acute rejection in 3 excised renal allograft of sensitized dogs. Seven days after operation, all sensitized dogs had lost graft function, pathologic changes of which showed that the renal allografts were seriously rejected. 2 of 3 dogs in control group were also acutely rejected.Conclusion:A convenient method by means of repeated stimulation of canine lymphocyte may induce specific immune sensitization in canine recipients. Renal allografts in sensitized dogs will be earlier rejected and result in a more deteriorated graft function.展开更多
文摘胃癌是我国的主要恶性肿瘤之一,其发病率及死亡率在恶性肿瘤中居于首位,严重影响人民的身体健康。近年来,虽然对胃癌的基础理论和临床作了大量的研究,但对胃癌的病因、生物学行为仍处在初步探索阶段,对早期胃癌的诊断还缺少客观化定量指标,治疗方法更是匮乏。因此,迫切需要对胃癌作更深入的研究。染色体在数量和结构上的畸变,是公认的一种癌细胞特征。DNA 是染色体的主要物质基础。正常胃粘膜细胞 DNA 含量较恒定,其倍体类型主要表现为二倍体。但由正常细胞经过转化细胞,直至变为癌细胞时。
文摘Objective:To establish a sensitized canine model for kidney transplantation.Methods:12 male dogs were averagely grouped as donors and recipients. A small number of donor canine lymphocytes was infused into different anatomic locations of a paired canine recipient for each time and which was repeated weekly. Specific immune sensitization was monitored by means of Complement Dependent Cytotoxicity (CDC) and Mixed Lymphocyte Culture (MLC) test. When CDC test conversed to be positive and MLC test showed a significant proliferation of reactive lymphocytes of canine recipients, the right kidneys of the paired dogs were excised and transplanted to each other concurrently. Injury of renal allograft function was scheduled determined by ECT dynamic kidney photography and pathologic investigation. Results:CDC test usually conversed to be positive and reactive lymphocytes of canine recipients were also observed to be proliferated significantly in MLC test after 3 to 4 times of canine donor lymphocyte infusions. Renal allograft function deterioration occurred 4 d post-operatively in 4 of 6 canine recipients, in contrast to none in control dogs. Pathologic changes suggested antibody-mediated rejection (delayed) or acute rejection in 3 excised renal allograft of sensitized dogs. Seven days after operation, all sensitized dogs had lost graft function, pathologic changes of which showed that the renal allografts were seriously rejected. 2 of 3 dogs in control group were also acutely rejected.Conclusion:A convenient method by means of repeated stimulation of canine lymphocyte may induce specific immune sensitization in canine recipients. Renal allografts in sensitized dogs will be earlier rejected and result in a more deteriorated graft function.
